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Binding was measured on brain tubulin, not on tubulin from a target tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mebendazole research collection; topical membership is not evidence of a direct clinical effect, and mebendazole is recorded separately from albendazole, from the benzimidazole class and from its own crystal forms.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mebendazole","display_name":"Mebendazole","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Mammal","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At body temperature the drug slides off our tubulin and locks onto the worm’s; cool ours down and it sticks to that too.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mbz-p1554382] Binding of [3H]benzimidazole carbamates to mammalian brain tubulin and the mechanism of selective toxicity of the benzimidazole anthelmintics. (1992). https://pubmed.ncbi.nlm.nih.gov/1554382/ DOI: 10.1016/0006-2952(92)90617-r","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain tubulin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"153562e8-d761-51c3-9f60-66a117856cb4","evidence_kind":"source_excerpt","locator":"Lines 160-171","start_line":160,"end_line":171,"excerpt":"### mbz-temperature-decides\nTritiated benzimidazole carbamate binding to mammalian brain tubulin was markedly greater at 4 degrees than at 37 degrees for all ligands, association constants and maximum binding were temperature dependent, and the first order dissociation rate constant of the mebendazole-tubulin complex was reduced by two orders of magnitude at 4 degrees compared with 37 degrees, suggesting that temperature-induced conformational changes in the tubulin dimer influence the ability to form a stable complex; this is unlike the interaction observed for parasitic nematodes where optimum binding occurs at 37 degrees and results in a pseudo-irreversible complex.\nCondition category: normal\nnutrient_topic: Mebendazole research collection; topical membership is not evidence of a direct clinical effect, and mebendazole is recorded separately from albendazole, from the benzimidazole class and from its own crystal forms.\nplain_language: At body temperature the drug slides off our tubulin and locks onto the worm’s; cool ours down and it sticks to that too.\norganism: Mammal\ntissue_or_cell_type: Brain tubulin\nexperimental_model: Tritiated benzimidazole carbamate binding to mammalian brain tubulin measured at 4 and 37 degrees\nlimitations: The temperature arm is what makes this decisive: it explains why the same drug behaves differently on host and parasite tubulin at body temperature. Binding was measured on brain tubulin, not on tubulin from a target tissue.\nexposure: Mebendazole, oxibendazole, oxfendazole and carbendazim binding measured across temperature\nevidence_span: {\"source_cache\": \"artifacts/mebendazole-research/1554382.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"70fec1c41b747eaae6d4a47ad1075a49828ee942f7935b85eb0f51416cd57c37\", \"start_char\": 0, \"end_char\": 1495, \"text_sha256\": \"70fec1c41b747eaae6d4a47ad1075a49828ee942f7935b85eb0f51416cd57c37\"}\n[mbz-p1554382] Binding of [3H]benzimidazole carbamates to mammalian brain tubulin and the mechanism of selective toxicity of the benzimidazole anthelmintics. 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