{"id":"90c30ef0-7656-5c75-a575-50d38d7f602b","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-xanthine-oxidase-prevention","predicate":"prevents","statement":"Exposure inhibited carbon monoxide-mediated brain lipid peroxidation by preventing the conversion of xanthine dehydrogenase to oxidase, a conversion known to be due to the action of leukocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0b0aba3f-a97d-57d1-9a4e-d4a4877ac3b1","mechanism_event_label":"Stopping the white cells sticking stops the enzyme switch that generates the damage.","subject":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"object":{"id":"0e429533-b216-5dd9-bd26-70ac9114b997","slug":"xanthine-oxidase-conversion","display_name":"Conversion of xanthine dehydrogenase to xanthine oxidase","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0b0aba3f-a97d-57d1-9a4e-d4a4877ac3b1","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-xanthine-oxidase-prevention-event","event_type":"biochemical_relationship","label":"Stopping the white cells sticking stops the enzyme switch that generates the damage.","description":"Exposure inhibited carbon monoxide-mediated brain lipid peroxidation by preventing the conversion of xanthine dehydrogenase to oxidase, a conversion known to be due to the action of leukocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8de34d8a-46dc-512c-a82f-d169747b496e","slug":"xdh","display_name":"Human xanthine oxidoreductase / XDH","entity_type_key":"protein"},"role":"affected_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab1a14a9-2c6b-5a60-b2ac-e65519277b78","slug":"brain-lipid-peroxidation","display_name":"Brain lipid peroxidation after carbon monoxide poisoning","entity_type_key":"cellular_process"},"role":"downstream_process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"581fa746-cb84-571b-87a0-4d461263f660","slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0e429533-b216-5dd9-bd26-70ac9114b997","slug":"xanthine-oxidase-conversion","display_name":"Conversion of xanthine dehydrogenase to xanthine oxidase","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/8248932.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\", \"start_char\": 0, \"end_char\": 1870, \"text_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats poisoned with carbon monoxide, with leukocyte function assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"3 atmospheres absolute for 45 minutes, given 24 hours before or up to 45 minutes after poisoning; 3 ATA pressure without oxygen as a control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The pressure-only control separates oxygen from pressure. The integrin defect is functional: surface expression was normal, which is the informative detail.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Rat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Stopping the white cells sticking stops the enzyme switch that generates the damage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8248932/ DOI: 10.1006/taap.1993.1243","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain microvasculature and polymorphonuclear leukocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e9a4bf1-4104-5a2a-a2ad-e9c10c9d75f6","evidence_kind":"source_excerpt","locator":"Lines 764-775","start_line":764,"end_line":775,"excerpt":"### hbot-xanthine-oxidase-prevention\nExposure inhibited carbon monoxide-mediated brain lipid peroxidation by preventing the conversion of xanthine dehydrogenase to oxidase, a conversion known to be due to the action of leukocytes.\nCondition category: normal\nnutrient_topic: Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.\nplain_language: Stopping the white cells sticking stops the enzyme switch that generates the damage.\norganism: Rat\ntissue_or_cell_type: Brain microvasculature and polymorphonuclear leukocytes\nexperimental_model: Rats poisoned with carbon monoxide, with leukocyte function assays\nlimitations: The pressure-only control separates oxygen from pressure. The integrin defect is functional: surface expression was normal, which is the informative detail.\nexposure: 3 atmospheres absolute for 45 minutes, given 24 hours before or up to 45 minutes after poisoning; 3 ATA pressure without oxygen as a control\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/8248932.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\", \"start_char\": 0, \"end_char\": 1870, \"text_sha256\": \"54ab057eb31320490d893896d8ab5b0c856a8b59489424b14b81409f01a78f7f\"}\n[hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8248932/ DOI: 10.1006/taap.1993.1243","model_system":"Rats poisoned with carbon monoxide, with leukocyte function assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hbot-p8248932] Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8248932/ DOI: 10.1006/taap.1993.1243","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dbf39b48-c95e-5291-b404-349ffbc4488f","stable_key":"import-488ea171-36b8-5ef6-b5d3-98563980fdbf","title":"Hyperbaric oxygen: the exposure, its reactive species, the signals they carry, and the nutrient-dependent enzymes that handle them (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"fb2443321804010d82c6b96ab61cc51b4b6628ddbb15751cb0e90b491606e79c","revision_id":"bb699ea5-b492-5728-b22c-48f0842a8ac9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}