{"id":"90a699ba-e11d-5358-8737-dc3a8cb424a6","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hmox1-induction","predicate":"increased_expression_of","statement":"Sulforaphane increased HMOX1 gene expression in early-passage nonsenescent MRC-5 fibroblasts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5619bc73-ecd3-5c8f-9a04-7e2177112a66","mechanism_event_label":"The response includes this separately identified defense-system protein.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"26298c7c-5265-5bf5-a435-e0fa98a3ac10","slug":"hmox1","display_name":"Human heme oxygenase 1 / HMOX1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"5619bc73-ecd3-5c8f-9a04-7e2177112a66","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hmox1-induction-event","event_type":"biochemical_relationship","label":"The response includes this separately identified defense-system protein.","description":"Sulforaphane increased HMOX1 gene expression in early-passage nonsenescent MRC-5 fibroblasts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"26298c7c-5265-5bf5-a435-e0fa98a3ac10","slug":"hmox1","display_name":"Human heme oxygenase 1 / HMOX1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/30595796.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"13f25a5ca61ca9bdf80d4b24878682c90b414a5718a73b6a5b86ff20a9673279\", \"start_char\": 14986, \"end_char\": 15205, \"text_sha256\": \"c8a5b844826129e5cff61e4e19c0eb34f04a29a8feb8ced52a567eae1585d935\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Replicative culture and metabolic/gene-expression measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane treatment during serial culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture senescence is not human longevity; expression is not necessarily flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MRC-5 and BJ fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The response includes this separately identified defense-system protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p30595796] Sulforaphane Delays Fibroblast Senescence by Curbing Cellular Glucose Uptake, Increased Glycolysis, and Oxidative Damage. (2018). https://pubmed.ncbi.nlm.nih.gov/30595796/ DOI: 10.1155/2018/5642148","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Glucose handling, antioxidant response and senescence","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e0aa885a-bcec-5d9b-b4d1-7b8ea270df99","evidence_kind":"source_excerpt","locator":"Lines 957-968","start_line":957,"end_line":968,"excerpt":"### sulforaphane-hmox1-induction\nSulforaphane increased HMOX1 gene expression in early-passage nonsenescent MRC-5 fibroblasts.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The response includes this separately identified defense-system protein.\norganism: Human MRC-5 and BJ fibroblasts\ntissue_or_cell_type: Glucose handling, antioxidant response and senescence\nexperimental_model: Replicative culture and metabolic/gene-expression measurements\nlimitations: Cell-culture senescence is not human longevity; expression is not necessarily flux.\nexposure: Sulforaphane treatment during serial culture\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/30595796.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"13f25a5ca61ca9bdf80d4b24878682c90b414a5718a73b6a5b86ff20a9673279\", \"start_char\": 14986, \"end_char\": 15205, \"text_sha256\": \"c8a5b844826129e5cff61e4e19c0eb34f04a29a8feb8ced52a567eae1585d935\"}\n[sulforaphane-p30595796] Sulforaphane Delays Fibroblast Senescence by Curbing Cellular Glucose Uptake, Increased Glycolysis, and Oxidative Damage. 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