{"id":"909b9790-f83b-5df7-b086-4479bc786d92","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars2-plp-intervention","predicate":"improves_tested","statement":"PLP treatment or an H2S donor improved brown-adipocyte function and reduced obesity progression in the reported high-fat-fed mouse experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ebdc1658-4fab-5ab6-9d90-208e970667cb","mechanism_event_label":"A B6-derived coenzyme and a sulfur donor were separately tested inputs to this pathway.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"b4551151-4548-5b96-ba40-da9dd46800b1","slug":"mouse-cars2-brown-fat-program","display_name":"Brown-fat development and function in mouse Cars2 experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ebdc1658-4fab-5ab6-9d90-208e970667cb","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars2-plp-intervention-event","event_type":"observed_relationship","label":"A B6-derived coenzyme and a sulfur donor were separately tested inputs to this pathway.","description":"PLP treatment or an H2S donor improved brown-adipocyte function and reduced obesity progression in the reported high-fat-fed mouse experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b4551151-4548-5b96-ba40-da9dd46800b1","slug":"mouse-cars2-brown-fat-program","display_name":"Brown-fat development and function in mouse Cars2 experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9fe61578-bc3d-54ef-947a-4537a63d47ba","slug":"mouse-cars2","display_name":"Mouse mitochondrial cysteinyl-tRNA synthetase / Cars2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"104876de-8ff6-5cf1-940f-1ef80bfbe85e","slug":"mouse-ebf2","display_name":"Mouse early B-cell factor 2 / Ebf2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse high-fat-diet and brown-adipocyte experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-only intervention details; no human dose, route equivalence or cysteine-plus-B6 synergy is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A B6-derived coenzyme and a sulfur donor were separately tested inputs to this pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41849685/ · DOI 10.1002/advs.202522690","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6222d145-2586-5408-b1b7-542077bbedeb","evidence_kind":"source_excerpt","locator":"Lines 412-418","start_line":412,"end_line":418,"excerpt":"## l-cysteine-cars2-plp-intervention\nA B6-derived coenzyme and a sulfur donor were separately tested inputs to this pathway.\nPLP treatment or an H2S donor improved brown-adipocyte function and reduced obesity progression in the reported high-fat-fed mouse experiments.\nModel: Mouse high-fat-diet and brown-adipocyte experiments.\nLimitations: Abstract-only intervention details; no human dose, route equivalence or cysteine-plus-B6 synergy is established.\nEvidence access: Primary abstract\nCars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41849685/ · DOI 10.1002/advs.202522690","model_system":"Mouse high-fat-diet and brown-adipocyte experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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