{"id":"904df174-0ee0-58ea-a3b0-3bf58256d996","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-cpla2-nfkb","predicate":"inhibits","statement":"Eugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5ae85c9b-330e-52ef-a497-8933d9e01b4e","mechanism_event_label":"Eugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"cbf994cd-fe9a-55fa-992d-e08de1bc9676","slug":"human-platelet-cpla2-nfkb-axis","display_name":"Human platelet cPLA2-to-NF-kappaB signaling axis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5ae85c9b-330e-52ef-a497-8933d9e01b4e","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-cpla2-nfkb-event","event_type":"observed_relationship","label":"Eugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.","description":"Eugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cbf994cd-fe9a-55fa-992d-e08de1bc9676","slug":"human-platelet-cpla2-nfkb-axis","display_name":"Human platelet cPLA2-to-NF-kappaB signaling axis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"875a94da-1eee-50d2-b5c1-e4316c2a8f45","slug":"nf-kappab-signaling","display_name":"NF-kappa-B signaling","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol with CAY10502 or BAY11-7082 pathway probes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Platelets from healthy human donors; mouse mesenteric-thrombosis arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Anucleate platelet NF-kappaB signaling and mouse plug formation do not establish chronic human outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Platelets from healthy human donors; mouse mesenteric-thrombosis arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Eugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Eugenol: A Potential Modulator of Human Platelet Activation and Mouse Mesenteric Vascular Thrombosis via an Innovative cPLA2-NF-κB Signaling Axis. (2024). https://pubmed.ncbi.nlm.nih.gov/39200154/ DOI: 10.3390/biomedicines12081689","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro and in vivo mouse model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"cPLA2 and NF-kappaB signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"313a788e-3fff-589b-8135-141518d1fd07","evidence_kind":"source_excerpt","locator":"Lines 33-42","start_line":33,"end_line":42,"excerpt":"## eugenol-cpla2-nfkb\nEugenol reduced collagen-stimulated IKK and p65 phosphorylation and I-kappaB-alpha degradation; inhibitor ordering placed cPLA2 upstream of NF-kappaB in human platelets.\nModel/species: Platelets from healthy human donors; mouse mesenteric-thrombosis arm\nTissue/system: cPLA2 and NF-kappaB signaling\nExposure: Eugenol with CAY10502 or BAY11-7082 pathway probes\nRoute: In vitro and in vivo mouse model\nDuration: Acute\nLimits: Anucleate platelet NF-kappaB signaling and mouse plug formation do not establish chronic human outcomes.\nPrimary reference: Eugenol: A Potential Modulator of Human Platelet Activation and Mouse Mesenteric Vascular Thrombosis via an Innovative cPLA2-NF-κB Signaling Axis. (2024). https://pubmed.ncbi.nlm.nih.gov/39200154/ DOI: 10.3390/biomedicines12081689\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Platelets from healthy human donors; mouse mesenteric-thrombosis arm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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