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(2007). https://pubmed.ncbi.nlm.nih.gov/17456783/ DOI: 10.1096/fj.07-8227com","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Primary hepatocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b5ccc15a-4a12-5714-8a7e-116ffb52dced","evidence_kind":"source_excerpt","locator":"Lines 828-839","start_line":828,"end_line":839,"excerpt":"### choline-estrogen-pemt-transcription\nEstradiol increased PEMT transcription in primary human hepatocytes in a dose-dependent manner.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Hormone status can affect endogenous choline-headgroup synthesis.\norganism: Human primary hepatocytes\ntissue_or_cell_type: Primary hepatocytes\nexperimental_model: Primary-hepatocyte estrogen exposure\nlimitations: Culture regulation supports a mechanism but does not set requirements for every person or justify hormone treatment.\nexposure: 17-beta-estradiol treatment for 24 hours\nevidence_span: {\"source_cache\": \"artifacts/choline-research/17456783.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d3585209a8c4db7cfe8b93aa2587fdd570f5e5c42bd3ea8f6195feb60b23b9da\", \"start_char\": 0, \"end_char\": 1399, \"text_sha256\": \"d3585209a8c4db7cfe8b93aa2587fdd570f5e5c42bd3ea8f6195feb60b23b9da\"}\n[choline-p17456783] Phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes. (2007). https://pubmed.ncbi.nlm.nih.gov/17456783/ DOI: 10.1096/fj.07-8227com","model_system":"Primary-hepatocyte estrogen exposure","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p17456783] Phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes. (2007). https://pubmed.ncbi.nlm.nih.gov/17456783/ DOI: 10.1096/fj.07-8227com","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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