{"id":"9033b40a-67f1-54d2-872b-5a7d84017343","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-calcium","predicate":"reduces_tested","statement":"Glycine at 1 mM nearly prevented the calcium rise elicited by 10 micrograms/mL LPS in cultured rat Kupffer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4f7a8b25-3b36-55b0-8793-9ca8463e799d","mechanism_event_label":"Glycine changed a calcium signal in liver immune cells.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"4085b7b6-7739-5044-aea7-8efe036023ae","slug":"rat-kupffer-lps-calcium","display_name":"LPS-evoked calcium rise in rat Kupffer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4f7a8b25-3b36-55b0-8793-9ca8463e799d","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-calcium-event","event_type":"observed_relationship","label":"Glycine changed a calcium signal in liver immune cells.","description":"Glycine at 1 mM nearly prevented the calcium rise elicited by 10 micrograms/mL LPS in cultured rat Kupffer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4085b7b6-7739-5044-aea7-8efe036023ae","slug":"rat-kupffer-lps-calcium","display_name":"LPS-evoked calcium rise in rat Kupffer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6d316e3b-ba0b-5161-a57e-59c2e3869810","slug":"rat-kupffer-glycine-chloride-response","display_name":"Chloride-dependent glycine response in rat Kupffer cells","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat Kupffer-cell culture with glycine, LPS and receptor pharmacology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High in-vitro exposure; not a demonstrated systemic anti-inflammatory effect in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glycine changed a calcium signal in liver immune cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Kupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"93594329-e53d-550f-9b3b-c77533c14f6e","evidence_kind":"source_excerpt","locator":"Lines 330-336","start_line":330,"end_line":336,"excerpt":"## glycine-kupffer-calcium\nGlycine changed a calcium signal in liver immune cells.\nGlycine at 1 mM nearly prevented the calcium rise elicited by 10 micrograms/mL LPS in cultured rat Kupffer cells.\nModel: Rat Kupffer-cell culture with glycine, LPS and receptor pharmacology.\nLimitations: High in-vitro exposure; not a demonstrated systemic anti-inflammatory effect in humans.\nEvidence access: Primary abstract\nKupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","model_system":"Rat Kupffer-cell culture with glycine, LPS and receptor pharmacology.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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