{"id":"901decb4-cfa2-5d60-91c5-c8d0eceed070","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-complex-assembly","predicate":"deletion_reduces","statement":"Cardiac Ndufab1 deletion reduced assembled respiratory complexes I–III and supercomplexes in mouse heart mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"27ea1daf-916d-53f7-9917-8d81215dad97","mechanism_event_label":"Loss of the carrier impaired assembly of several respiratory complexes in mouse heart.","subject":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"object":{"id":"b7871450-a775-565c-94b3-72e8eca0966f","slug":"respiratory-supercomplex-assembly","display_name":"Mitochondrial respiratory supercomplex assembly","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"27ea1daf-916d-53f7-9917-8d81215dad97","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-complex-assembly-event","event_type":"biochemical_relationship","label":"Loss of the carrier impaired assembly of several respiratory complexes in mouse heart.","description":"Cardiac Ndufab1 deletion reduced assembled respiratory complexes I–III and supercomplexes in mouse heart mitochondria.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"affected_complex","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4dc2faa3-f38c-5813-b7f2-fe19a0ab7bfa","slug":"respiratory-complex-ii","display_name":"Respiratory complex II","entity_type_key":"protein_complex"},"role":"affected_complex","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3b73f316-a551-517d-82b8-6af922d5a091","slug":"respiratory-complex-iii","display_name":"Mitochondrial respiratory complex III","entity_type_key":"protein_complex"},"role":"affected_complex","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"682b6b9c-ffde-5ba0-bccf-fb05de6e6002","slug":"mouse-sdhb","display_name":"Mouse succinate dehydrogenase iron-sulfur subunit / Sdhb","entity_type_key":"protein"},"role":"affected_FeS_subunit","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c78cfc58-7c63-5951-aa14-56afeb975e06","slug":"mouse-uqcrfs1","display_name":"Mouse complex III Rieske iron-sulfur subunit / Uqcrfs1","entity_type_key":"protein"},"role":"affected_FeS_subunit","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"b7871450-a775-565c-94b3-72e8eca0966f","slug":"respiratory-supercomplex-assembly","display_name":"Mitochondrial respiratory supercomplex assembly","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 49–55; Fig. 3; Supplementary Figs. S6–S10","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cardiac-specific genetic deletion; 6- and 16-week assembly assays, 6- and 10-week respiratory assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The FeS subunits of complexes II/III were preferentially affected, whereas broader complex-I subunit loss supports an additional assembly role. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of the carrier impaired assembly of several respiratory complexes in mouse heart.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"393cdfb3-c70b-5f26-87c8-5b8e7a07a0ed","evidence_kind":"source_excerpt","locator":"Lines 949-961","start_line":949,"end_line":961,"excerpt":"### b5-met-heart-acp-complex-assembly\nCardiac Ndufab1 deletion reduced assembled respiratory complexes I–III and supercomplexes in mouse heart mitochondria.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of the carrier impaired assembly of several respiratory complexes in mouse heart.\norganism: Mus musculus\ntissue_or_cell_type: Heart mitochondria\nexperimental_model: Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria\nlimitations: The FeS subunits of complexes II/III were preferentially affected, whereas broader complex-I subunit loss supports an additional assembly role. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Cardiac-specific genetic deletion; 6- and 16-week assembly assays, 6- and 10-week respiratory assays.\ncross_nutrient: true\nevidence_location: Full text lines 49–55; Fig. 3; Supplementary Figs. S6–S10\n[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","model_system":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}