{"id":"90078ccf-b5c3-5a24-b74a-059b86c27064","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-sit1-glycine","predicate":"also_transports","statement":"Human SIT1 produced glycine-evoked current about 18% of the proline response in the reported substrate comparison; sarcosine current was about 83%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ec41b904-5b5f-53aa-a99f-cecc94322b0e","mechanism_event_label":"This transporter has overlapping amino-acid routes with unequal efficiency.","subject":{"id":"01b4d4c1-f1a8-59e3-84e3-fb851d5ddf46","slug":"slc6a20","display_name":"Human imino-acid transporter / SLC6A20","entity_type_key":"protein"},"object":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ec41b904-5b5f-53aa-a99f-cecc94322b0e","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-sit1-glycine-event","event_type":"observed_relationship","label":"This transporter has overlapping amino-acid routes with unequal efficiency.","description":"Human SIT1 produced glycine-evoked current about 18% of the proline response in the reported substrate comparison; sarcosine current was about 83%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"01b4d4c1-f1a8-59e3-84e3-fb851d5ddf46","slug":"slc6a20","display_name":"Human imino-acid transporter / SLC6A20","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SIT1 in Xenopus oocytes; 1 mM substrates at pH 7.4 and -60 mV.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Failure of glycine to stabilize purified SIT1 thermally did not rule out its transport; binding-proxy and transport assays measure different things.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This transporter has overlapping amino-acid routes with unequal efficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38951531/ · DOI 10.1038/s41467-024-48921-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"787f564f-7042-572d-8191-d2492da4f4c5","evidence_kind":"source_excerpt","locator":"Lines 190-196","start_line":190,"end_line":196,"excerpt":"## l-proline-sit1-glycine\nThis transporter has overlapping amino-acid routes with unequal efficiency.\nHuman SIT1 produced glycine-evoked current about 18% of the proline response in the reported substrate comparison; sarcosine current was about 83%.\nModel: Human SIT1 in Xenopus oocytes; 1 mM substrates at pH 7.4 and -60 mV.\nLimitations: Failure of glycine to stabilize purified SIT1 thermally did not rule out its transport; binding-proxy and transport assays measure different things.\nEvidence access: Primary full text\nStructure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38951531/ · DOI 10.1038/s41467-024-48921-x","model_system":"Human SIT1 in Xenopus oocytes; 1 mM substrates at pH 7.4 and -60 mV.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}