{"id":"8fe05f3a-87a4-57f3-83cc-08b9a99b9df0","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-lacphe-genetic","predicate":"supports","statement":"Global Cndp2 knockout markedly reduced circulating Lac-Phe and attenuated the food-intake and weight response to chronic exercise on a high-fat diet.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8da6cc3e-a285-5c53-a551-287bc9dc6832","mechanism_event_label":"Removing a production enzyme weakened one part of the exercise response.","subject":{"id":"44b44508-9e2c-50ee-a9ac-81b2b009641e","slug":"mouse-cndp2","display_name":"Mouse cytosolic nonspecific dipeptidase / Cndp2","entity_type_key":"protein"},"object":{"id":"668bfd19-3032-525d-a5b5-8bdeb100591e","slug":"mouse-exercise-lacphe-feeding","display_name":"Mouse exercise-associated Lac-Phe and feeding regulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8da6cc3e-a285-5c53-a551-287bc9dc6832","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-lacphe-genetic-event","event_type":"observed_relationship","label":"Removing a production enzyme weakened one part of the exercise response.","description":"Global Cndp2 knockout markedly reduced circulating Lac-Phe and attenuated the food-intake and weight response to chronic exercise on a high-fat diet.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"44b44508-9e2c-50ee-a9ac-81b2b009641e","slug":"mouse-cndp2","display_name":"Mouse cytosolic nonspecific dipeptidase / Cndp2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"668bfd19-3032-525d-a5b5-8bdeb100591e","slug":"mouse-exercise-lacphe-feeding","display_name":"Mouse exercise-associated Lac-Phe and feeding regulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2e1e9f48-df5d-5d8a-a1e9-96e90a6eb6d1","slug":"n-lactoyl-phenylalanine","display_name":"N-Lactoyl-phenylalanine / Lac-Phe","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse global Cndp2 knockout; repeated treadmill exercise with high-fat feeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cndp2 has other substrates; the phenotype was conditional on exercise and diet and did not establish a human deficiency syndrome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing a production enzyme weakened one part of the exercise response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"An exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"934b68ad-a935-5ce8-ae7e-2c74b7887a90","evidence_kind":"source_excerpt","locator":"Lines 414-420","start_line":414,"end_line":420,"excerpt":"## l-phenylalanine-lacphe-genetic\nRemoving a production enzyme weakened one part of the exercise response.\nGlobal Cndp2 knockout markedly reduced circulating Lac-Phe and attenuated the food-intake and weight response to chronic exercise on a high-fat diet.\nModel: Mouse global Cndp2 knockout; repeated treadmill exercise with high-fat feeding.\nLimitations: Cndp2 has other substrates; the phenotype was conditional on exercise and diet and did not establish a human deficiency syndrome.\nEvidence access: Primary full text\nAn exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","model_system":"Mouse global Cndp2 knockout; repeated treadmill exercise with high-fat feeding.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4d53adcd4acc7a9aecf44ccb2623ec540c9d221a0f7c00002585870b39b8ffb8","revision_id":"fd182a81-4ab7-559a-9c77-fb4cf72982ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}