{"id":"8f6fb829-72bb-5918-95b2-fa9a64c92c85","stable_key":"9ee3c90a-c675-5dce-a105-4dc6909dfeab:ifnar-loss-apt2-transcript","predicate":"has_contextual_experimental_result","statement":"IFNAR deficiency reduced infected-red-cell-induced APT2 transcript expression in peritoneal macrophages.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d7df37f9-0df4-59ea-aeaa-9ec0ff93a4f0","mechanism_event_label":"ifnar-loss-apt2-transcript","subject":{"id":"5d3e33cc-84a0-5d44-a1a8-f6536fee9b9e","slug":"mouse-ifnar-deficient-pems","display_name":"Mouse IFNAR-deficient peritoneal macrophages","entity_type_key":"protein_state"},"object":{"id":"eadf4363-4726-5e71-9c54-e11cccaf722d","slug":"mouse-apt2-transcript-abundance","display_name":"APT2 transcript abundance in mouse cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d7df37f9-0df4-59ea-aeaa-9ec0ff93a4f0","stable_key":"9ee3c90a-c675-5dce-a105-4dc6909dfeab:ifnar-loss-apt2-transcript","event_type":"experimental_result","label":"ifnar-loss-apt2-transcript","description":"IFNAR deficiency reduced infected-red-cell-induced APT2 transcript expression in peritoneal macrophages.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5d3e33cc-84a0-5d44-a1a8-f6536fee9b9e","slug":"mouse-ifnar-deficient-pems","display_name":"Mouse IFNAR-deficient peritoneal macrophages","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eadf4363-4726-5e71-9c54-e11cccaf722d","slug":"mouse-apt2-transcript-abundance","display_name":"APT2 transcript abundance in mouse cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1345a511-9164-5363-ad1e-5b0212c0259d","slug":"mouse-lypla2","display_name":"Mouse acyl-protein thioesterase 2 / LYPLA2","entity_type_key":"protein"},"role":"transcript-encoded protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Mouse wild-type versus Ifnar-deficient peritoneal exudate macrophages","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Freeze-thawed Plasmodium yoelii N67 infected red blood cells at 0, 6, 12 and 24 hours (Figure 7d visually reviewed).","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The manuscript calls the genotype Ifnar-deficient; this extraction does not assign a specific disrupted subunit allele. No specific IFNAR1 or IFNAR2 state link is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 7d; RT-qPCR and Results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-025-65081-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"afad3ccf-a46b-55e4-b46c-e6d0dbc429f3","evidence_kind":"source_excerpt","locator":"Lines 96-102","start_line":96,"end_line":102,"excerpt":"IFNAR deficiency reduced infected-red-cell-induced APT2 transcript expression in peritoneal macrophages.\nprimary_references: https://doi.org/10.1038/s41467-025-65081-8\nprimary_locator: Figure 7d; RT-qPCR and Results.\norganism: Mouse\nexperimental_model: Mouse wild-type versus Ifnar-deficient peritoneal exudate macrophages\nexposure: Freeze-thawed Plasmodium yoelii N67 infected red blood cells at 0, 6, 12 and 24 hours (Figure 7d visually reviewed).\nlimitations: The manuscript calls the genotype Ifnar-deficient; this extraction does not assign a specific disrupted subunit allele. No specific IFNAR1 or IFNAR2 state link is inferred.","model_system":"Mouse wild-type versus Ifnar-deficient peritoneal exudate macrophages","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of primary result and assay context.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dcc74980-d350-5911-b545-8c47ecdcf8fd","stable_key":"import-9ee3c90a-c675-5dce-a105-4dc6909dfeab","title":"MAVS inhibitor nulls and APT2-TBK1 immune feedback","document_type":"imported_text","citation_label":"Selected primary observations: 10.1073/pnas.2403392121; 10.1038/s41467-025-65081-8.","file_path":"","sha256":"16b3ee62ea6e0331133d1f7e9adbf7569a58fcbfda2b64c25a12f0dc4cdf4fe6","revision_id":"a5ea594c-6318-5060-b1c7-26e57336aa8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}