{"id":"8f3aad7b-503c-59c9-9d9c-ac58c211c1f4","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-cardiac-calcium","predicate":"increases","statement":"Vanadate-treated rat cardiomyocytes showed sustained elevation of cytosolic calcium after 24 hours near the viability-loss concentrations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f4229daa-3a46-548d-89ac-607e99ecf27a","mechanism_event_label":"Ion regulation changed alongside mitochondrial injury.","subject":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"object":{"id":"fe19ac90-48ed-5e6f-925e-1c5f85ea65a4","slug":"rat-vanadate-cytosolic-calcium","display_name":"Cytosolic calcium in vanadate-exposed rat cardiomyocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f4229daa-3a46-548d-89ac-607e99ecf27a","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-cardiac-calcium-event","event_type":"observed_relationship","label":"Ion regulation changed alongside mitochondrial injury.","description":"Vanadate-treated rat cardiomyocytes showed sustained elevation of cytosolic calcium after 24 hours near the viability-loss concentrations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab633bfc-d259-5deb-ac81-a3383f79d4bf","slug":"decavanadate","display_name":"Decavanadate(V)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fe19ac90-48ed-5e6f-925e-1c5f85ea65a4","slug":"rat-vanadate-cytosolic-calcium","display_name":"Cytosolic calcium in vanadate-exposed rat cardiomyocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5acecb4f-d4c7-5549-8950-bf6e2085349d","slug":"metavanadate-assay-solution","display_name":"Metavanadate assay solution with mixed oligomers","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fura-2 imaging in neonatal rat cardiomyocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Association does not identify whether SERCA, other transporters or secondary cell injury caused the calcium rise.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Ion regulation changed alongside mitochondrial injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Vanadate induces necrotic death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18251508/ · DOI 10.1021/tx700204r","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c423a77-321f-5891-8dfe-f892b8906f3f","evidence_kind":"source_excerpt","locator":"Lines 302-308","start_line":302,"end_line":308,"excerpt":"## vanadium-cardiac-calcium\nIon regulation changed alongside mitochondrial injury.\nVanadate-treated rat cardiomyocytes showed sustained elevation of cytosolic calcium after 24 hours near the viability-loss concentrations.\nModel: Fura-2 imaging in neonatal rat cardiomyocytes.\nLimitations: Association does not identify whether SERCA, other transporters or secondary cell injury caused the calcium rise.\nEvidence access: Primary abstract\nVanadate induces necrotic death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18251508/ · DOI 10.1021/tx700204r","model_system":"Fura-2 imaging in neonatal rat cardiomyocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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