{"id":"8f2615b0-a4bf-5682-90f5-f02df1806711","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-colon-sodium","predicate":"undergoes_sodium_dependent_uptake","statement":"Biotin uptake in human NCM460 cells was saturable, energy dependent and sodium dependent, with apparent Km 19.7 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"80601824-eba9-572a-9d26-420490bd4c5c","mechanism_event_label":"Colon cells can take up free biotin through a saturable transport process.","subject":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"object":{"id":"936d4afd-d689-5a6f-a634-d4833de478dd","slug":"human-colonic-biotin-uptake","display_name":"Human colonic epithelial biotin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"80601824-eba9-572a-9d26-420490bd4c5c","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-colon-sodium-event","event_type":"biochemical_relationship","label":"Colon cells can take up free biotin through a saturable transport process.","description":"Biotin uptake in human NCM460 cells was saturable, energy dependent and sodium dependent, with apparent Km 19.7 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"coupled ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"936d4afd-d689-5a6f-a634-d4833de478dd","slug":"human-colonic-biotin-uptake","display_name":"Human colonic epithelial biotin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/9814986.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7cc679e31b95ae919c15fadf0d56933d2ed8074664e11cd80a96e910181857be\", \"start_char\": 0, \"end_char\": 1676, \"text_sha256\": \"7cc679e31b95ae919c15fadf0d56933d2ed8074664e11cd80a96e910181857be\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transport and inhibitor experiments in human NCM460 colonic epithelial cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Radiolabeled biotin with sodium replacement, pantothenate and kinase modulators","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line kinetics do not quantify microbiome contributions or demonstrate competition at ordinary dietary intakes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Colon cells can take up free biotin through a saturable transport process.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p9814986] Biotin uptake by human colonic epithelial NCM460 cells: a carrier-mediated process shared with pantothenic acid. (1998). https://pubmed.ncbi.nlm.nih.gov/9814986/ DOI: 10.1152/ajpcell.1998.275.5.c1365","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Colonic epithelium; NCM460 culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bd96b589-4f47-5290-9a55-aeb97f1fba2f","evidence_kind":"source_excerpt","locator":"Lines 182-193","start_line":182,"end_line":193,"excerpt":"### b7-colon-sodium\nBiotin uptake in human NCM460 cells was saturable, energy dependent and sodium dependent, with apparent Km 19.7 micromolar.\nCondition category: normal\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Colon cells can take up free biotin through a saturable transport process.\norganism: Homo sapiens\ntissue_or_cell_type: Colonic epithelium; NCM460 culture\nexperimental_model: Transport and inhibitor experiments in human NCM460 colonic epithelial cells\nlimitations: Cell-line kinetics do not quantify microbiome contributions or demonstrate competition at ordinary dietary intakes.\nexposure: Radiolabeled biotin with sodium replacement, pantothenate and kinase modulators\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/9814986.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7cc679e31b95ae919c15fadf0d56933d2ed8074664e11cd80a96e910181857be\", \"start_char\": 0, \"end_char\": 1676, \"text_sha256\": \"7cc679e31b95ae919c15fadf0d56933d2ed8074664e11cd80a96e910181857be\"}\n[b7-p9814986] Biotin uptake by human colonic epithelial NCM460 cells: a carrier-mediated process shared with pantothenic acid. (1998). https://pubmed.ncbi.nlm.nih.gov/9814986/ DOI: 10.1152/ajpcell.1998.275.5.c1365","model_system":"Transport and inhibitor experiments in human NCM460 colonic epithelial cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p9814986] Biotin uptake by human colonic epithelial NCM460 cells: a carrier-mediated process shared with pantothenic acid. (1998). https://pubmed.ncbi.nlm.nih.gov/9814986/ DOI: 10.1152/ajpcell.1998.275.5.c1365","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9608806b-adb6-5a35-b042-057147135642","stable_key":"import-08ce9896-9d1c-5bbf-b705-5bfe771091d5","title":"Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a05b23a45e0813ba2fcda0027e3f5d9d58b82858f8dd8598ffb7aca17e942a38","revision_id":"e0d0c2a2-e9e2-55dd-b467-41c22ba960d4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}