{"id":"8f13b41d-f6fa-53f3-8f37-24c29a958b52","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-pip5k-i5","predicate":"phosphorylates","statement":"Human PIP5K1C_i5 also displayed PI4P 5-kinase activity in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"915e6e06-83b9-5186-b685-7408248333ee","mechanism_event_label":"A separately identified isoform can carry out the same chemical step.","subject":{"id":"876046b0-aace-5814-b299-73bdf67abb36","slug":"pip5k1c-i5","display_name":"Human PIP5K1C_i5 isoform (707 amino acids)","entity_type_key":"protein"},"object":{"id":"da5bb942-9bd6-5e29-bc14-1f1df8007caa","slug":"phosphatidylinositol-4-phosphate","display_name":"Phosphatidylinositol 4-phosphate (PI4P)","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"915e6e06-83b9-5186-b685-7408248333ee","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-pip5k-i5-event","event_type":"biochemical_relationship","label":"A separately identified isoform can carry out the same chemical step.","description":"Human PIP5K1C_i5 also displayed PI4P 5-kinase activity in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"af38e50f-cdfb-5d4b-a6fb-f742d1b23d41","slug":"phosphatidylinositol-4-5-bisphosphate","display_name":"Phosphatidylinositol 4,5-bisphosphate","entity_type_key":"lipid"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphate_donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"876046b0-aace-5814-b299-73bdf67abb36","slug":"pip5k1c-i5","display_name":"Human PIP5K1C_i5 isoform (707 amino acids)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da5bb942-9bd6-5e29-bc14-1f1df8007caa","slug":"phosphatidylinositol-4-phosphate","display_name":"Phosphatidylinositol 4-phosphate (PI4P)","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/19548880.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5ae150d47bf139d7352c8a5a128e0d6b071340f8c47d78700d5f398a549d3cd8\", \"start_char\": 0, \"end_char\": 1766, \"text_sha256\": \"5ae150d47bf139d7352c8a5a128e0d6b071340f8c47d78700d5f398a549d3cd8\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human splice-isoform expression, localization and kinase assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PIP5K1C_i4 and i5 characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isoforms have different localizations; a bulk lipid concentration cannot describe every local pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human proteins in cellular expression assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A separately identified isoform can carry out the same chemical step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p19548880] Two novel phosphatidylinositol-4-phosphate 5-kinase type Igamma splice variants expressed in human cells display distinctive cellular targeting. (2009). https://pubmed.ncbi.nlm.nih.gov/19548880/ DOI: 10.1042/bj20090638","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Nuclear and vesicular compartments","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"623be14c-ac7f-5fc5-8d4e-1e844bdf8bb5","evidence_kind":"source_excerpt","locator":"Lines 587-598","start_line":587,"end_line":598,"excerpt":"### ino-pip5k-i5\nHuman PIP5K1C_i5 also displayed PI4P 5-kinase activity in vitro.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A separately identified isoform can carry out the same chemical step.\norganism: Human proteins in cellular expression assays\ntissue_or_cell_type: Nuclear and vesicular compartments\nexperimental_model: Human splice-isoform expression, localization and kinase assay\nlimitations: Isoforms have different localizations; a bulk lipid concentration cannot describe every local pool.\nexposure: PIP5K1C_i4 and i5 characterization\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/19548880.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5ae150d47bf139d7352c8a5a128e0d6b071340f8c47d78700d5f398a549d3cd8\", \"start_char\": 0, \"end_char\": 1766, \"text_sha256\": \"5ae150d47bf139d7352c8a5a128e0d6b071340f8c47d78700d5f398a549d3cd8\"}\n[ino-p19548880] Two novel phosphatidylinositol-4-phosphate 5-kinase type Igamma splice variants expressed in human cells display distinctive cellular targeting. (2009). https://pubmed.ncbi.nlm.nih.gov/19548880/ DOI: 10.1042/bj20090638","model_system":"Human splice-isoform expression, localization and kinase assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p19548880] Two novel phosphatidylinositol-4-phosphate 5-kinase type Igamma splice variants expressed in human cells display distinctive cellular targeting. (2009). https://pubmed.ncbi.nlm.nih.gov/19548880/ DOI: 10.1042/bj20090638","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}