{"id":"8eedacd8-18f2-54e1-82b8-fb0caf6eab93","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-trpv3","predicate":"activates","statement":"Eugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"dda6a679-8a07-52b6-843f-73e0fc94b291","mechanism_event_label":"Eugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"72b64948-783b-51ea-b7e6-5baa6256c849","slug":"human-trpv3","display_name":"Human transient receptor potential vanilloid 3 / TRPV3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"dda6a679-8a07-52b6-843f-73e0fc94b291","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-trpv3-event","event_type":"observed_relationship","label":"Eugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.","description":"Eugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"72b64948-783b-51ea-b7e6-5baa6256c849","slug":"human-trpv3","display_name":"Human transient receptor potential vanilloid 3 / TRPV3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol and related plant flavorants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"TRP-expressing cells and skin keratinocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Channel activation can contribute to chemesthesis or irritation and is not evidence of a therapeutic skin effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"TRP-expressing cells and skin keratinocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Eugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Oregano, thyme and clove-derived flavors and skin sensitizers activate specific TRP channels. (2006). https://pubmed.ncbi.nlm.nih.gov/16617338/ DOI: 10.1038/nn1692","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"TRPV3 channel activation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4cefa4bb-8409-530b-b1ea-c8883fa5d29d","evidence_kind":"source_excerpt","locator":"Lines 77-86","start_line":77,"end_line":86,"excerpt":"## eugenol-trpv3\nEugenol activated TRPV3 in heterologous and keratinocyte assays alongside related clove, oregano and thyme flavor compounds.\nModel/species: TRP-expressing cells and skin keratinocytes\nTissue/system: TRPV3 channel activation\nExposure: Eugenol and related plant flavorants\nRoute: In vitro\nDuration: Acute\nLimits: Channel activation can contribute to chemesthesis or irritation and is not evidence of a therapeutic skin effect.\nPrimary reference: Oregano, thyme and clove-derived flavors and skin sensitizers activate specific TRP channels. (2006). https://pubmed.ncbi.nlm.nih.gov/16617338/ DOI: 10.1038/nn1692\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"TRP-expressing cells and skin keratinocytes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}