{"id":"8ee33952-0bcd-54b7-a2fb-fc88d05d2b19","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-mfsd2a-uptake","predicate":"reduces","statement":"Mfsd2a-null mice had markedly reduced uptake of plasma-labeled LPC-DHA into the brain.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"489fcd0a-1c9e-5e29-aa9d-8fea3e7fe4c0","mechanism_event_label":"Less of the carrier lipid reached the brain.","subject":{"id":"69e20733-3bc8-5e19-afc0-c879ebbaaf8b","slug":"mouse-mfsd2a-null","display_name":"Experimental mouse Mfsd2a-null genotype","entity_type_key":"protein_state"},"object":{"id":"d6d11970-33b6-55ec-a360-b065711eeee7","slug":"mouse-brain-lpc-uptake","display_name":"Mouse plasma-to-brain LPC-DHA uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"489fcd0a-1c9e-5e29-aa9d-8fea3e7fe4c0","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-mfsd2a-uptake-event","event_type":"biochemical_relationship","label":"Less of the carrier lipid reached the brain.","description":"Mfsd2a-null mice had markedly reduced uptake of plasma-labeled LPC-DHA into the brain.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"69e20733-3bc8-5e19-afc0-c879ebbaaf8b","slug":"mouse-mfsd2a-null","display_name":"Experimental mouse Mfsd2a-null genotype","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d6d11970-33b6-55ec-a360-b065711eeee7","slug":"mouse-brain-lpc-uptake","display_name":"Mouse plasma-to-brain LPC-DHA uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/24828044.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3ec710edae591ed103960d73b49918a61b0260b22702926792e7133337b93695\", \"start_char\": 0, \"end_char\": 1689, \"text_sha256\": \"3ec710edae591ed103960d73b49918a61b0260b22702926792e7133337b93695\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse knockout and cell transport experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"LPC-bound versus unesterified DHA; sodium-dependent transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"LPC-DHA is a choline-containing lipid, not free choline. No claim that oral choline necessarily raises brain DHA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse Mfsd2a and experimental expression system","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Less of the carrier lipid reached the brain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p24828044] Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. (2014). https://pubmed.ncbi.nlm.nih.gov/24828044/ DOI: 10.1038/nature13241","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Blood-brain-barrier endothelium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1587fc06-d134-5a45-a4e7-528ac846f605","evidence_kind":"source_excerpt","locator":"Lines 971-982","start_line":971,"end_line":982,"excerpt":"### choline-mfsd2a-uptake\nMfsd2a-null mice had markedly reduced uptake of plasma-labeled LPC-DHA into the brain.\nCondition category: machinery_impairment\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Less of the carrier lipid reached the brain.\norganism: Mouse Mfsd2a and experimental expression system\ntissue_or_cell_type: Blood-brain-barrier endothelium\nexperimental_model: Mouse knockout and cell transport experiments\nlimitations: LPC-DHA is a choline-containing lipid, not free choline. No claim that oral choline necessarily raises brain DHA.\nexposure: LPC-bound versus unesterified DHA; sodium-dependent transport\nevidence_span: {\"source_cache\": \"artifacts/choline-research/24828044.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3ec710edae591ed103960d73b49918a61b0260b22702926792e7133337b93695\", \"start_char\": 0, \"end_char\": 1689, \"text_sha256\": \"3ec710edae591ed103960d73b49918a61b0260b22702926792e7133337b93695\"}\n[choline-p24828044] Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. (2014). https://pubmed.ncbi.nlm.nih.gov/24828044/ DOI: 10.1038/nature13241","model_system":"Mouse knockout and cell transport experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p24828044] Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid. (2014). https://pubmed.ncbi.nlm.nih.gov/24828044/ DOI: 10.1038/nature13241","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c7afe3d579eba0df6d2a4c26699c653462749e03188348f900c07748cc7282a8","revision_id":"0fa7b48f-2a3c-5adc-b13e-48727dd6f815","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}