{"id":"8ec6dcdf-b340-59f8-816b-c433d5dc4d38","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-rabbit-plc-pkc","predicate":"activates_pathway","statement":"Strontium-induced rabbit osteoclast apoptosis required PLC-dependent signaling and PKC beta II activation, with nuclear NF-kappaB translocation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a0311173-06a1-568c-9def-f18995596283","mechanism_event_label":"The shared calcium sensor can use a distinct downstream pathway.","subject":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"object":{"id":"a32d49cc-0f6d-5767-ba3e-cc0768839bea","slug":"rabbit-prkcb-beta2","display_name":"Rabbit protein kinase C beta II","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a0311173-06a1-568c-9def-f18995596283","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-rabbit-plc-pkc-event","event_type":"observed_relationship","label":"The shared calcium sensor can use a distinct downstream pathway.","description":"Strontium-induced rabbit osteoclast apoptosis required PLC-dependent signaling and PKC beta II activation, with nuclear NF-kappaB translocation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a32d49cc-0f6d-5767-ba3e-cc0768839bea","slug":"rabbit-prkcb-beta2","display_name":"Rabbit protein kinase C beta II","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bc93fef2-780a-5e1d-b4c7-24a087c7581b","slug":"rabbit-casr","display_name":"Rabbit calcium-sensing receptor / CaSR","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"1468da0e-e1a4-5b37-9b84-b3fe8d6cb075","slug":"rabbit-phospholipase-c-activity","display_name":"Rabbit osteoclast phospholipase C activity","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"35aba475-0d0e-5063-8ccc-a93f3e4638e2","slug":"rabbit-mature-osteoclast-apoptosis","display_name":"Apoptosis of mature rabbit osteoclasts","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rabbit mature-osteoclast signaling and inhibitor experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Accessed abstract does not resolve PLC or NF-kappaB subunits; do not assign human proteins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The shared calcium sensor can use a distinct downstream pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The calcium-sensing receptor is involved in strontium ranelate-induced osteoclast apoptosis. New insights into the associated signaling pathways. · 2009 · https://pubmed.ncbi.nlm.nih.gov/18927086/ · DOI 10.1074/jbc.M801668200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5420be62-70f1-5f82-8dff-aa13c6c752e6","evidence_kind":"source_excerpt","locator":"Lines 222-228","start_line":222,"end_line":228,"excerpt":"## strontium-rabbit-plc-pkc\nThe shared calcium sensor can use a distinct downstream pathway.\nStrontium-induced rabbit osteoclast apoptosis required PLC-dependent signaling and PKC beta II activation, with nuclear NF-kappaB translocation.\nModel: Rabbit mature-osteoclast signaling and inhibitor experiments.\nLimitations: Accessed abstract does not resolve PLC or NF-kappaB subunits; do not assign human proteins.\nEvidence access: Primary abstract\nThe calcium-sensing receptor is involved in strontium ranelate-induced osteoclast apoptosis. New insights into the associated signaling pathways. · 2009 · https://pubmed.ncbi.nlm.nih.gov/18927086/ · DOI 10.1074/jbc.M801668200","model_system":"Rabbit mature-osteoclast signaling and inhibitor experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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