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(2023). https://pubmed.ncbi.nlm.nih.gov/37585292/ DOI: 10.1016/j.celrep.2023.112982","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RPE microsomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"981c2351-fbb9-5a58-9239-8ede48497592","evidence_kind":"source_excerpt","locator":"Lines 498-511","start_line":498,"end_line":511,"excerpt":"### vae-rgr-photoproduct-release\nAfter photoisomerization in native bovine RPE microsomes, the RGR 11-cis-retinylidene Schiff base hydrolyzed with an estimated 7.5-second half-life at 20 degrees Celsius.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RGR released regenerated retinal within seconds in the membrane preparation.\norganism: Bos taurus\ntissue_or_cell_type: RPE microsomes\nexperimental_model: LC-MS/MS and retinoid analysis of native microsomes\nlimitations: Temperature-specific membrane kinetics do not directly specify a human whole-eye rate.\nexposure: 20 micromolar all-trans-retinal; 10-second 530-nm exposure, followed by dark incubation at 20 degrees Celsius.\ncross_nutrient: false\nevidence_location: Figure 1D-F; STAR Methods: hydrolysis kinetics\nnutrient: Vitamin A\n[vav-tworak2023] Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia. 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