{"id":"8e9d17b0-5d68-5c3f-8f7d-8c173ede969f","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-agat-reaction","predicate":"catalyzes_formation_of","statement":"AGAT catalyzes transfer of an amidino group from arginine to glycine, forming guanidinoacetate and ornithine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"eff38d5f-5df5-5eef-97ae-b6e86bc85f3f","mechanism_event_label":"The first synthesis step joins material from arginine and glycine to make the precursor of creatine.","subject":{"id":"c8ab5c45-4253-5dd5-be9c-2fcdfa69ac3a","slug":"gatm","display_name":"Human glycine amidinotransferase AGAT / GATM","entity_type_key":"protein"},"object":{"id":"84053a83-8fcc-5068-8df9-58f7e97fad76","slug":"guanidinoacetate","display_name":"Guanidinoacetate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"eff38d5f-5df5-5eef-97ae-b6e86bc85f3f","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-agat-reaction-event","event_type":"biochemical_relationship","label":"The first synthesis step joins material from arginine and glycine to make the precursor of creatine.","description":"AGAT catalyzes transfer of an amidino group from arginine to glycine, forming guanidinoacetate and ornithine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"amidino_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"amidino_acceptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c715e48e-42db-5fac-b4a4-c1285a68d085","slug":"ornithine","display_name":"L-Ornithine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"downstream_product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c8ab5c45-4253-5dd5-be9c-2fcdfa69ac3a","slug":"gatm","display_name":"Human glycine amidinotransferase AGAT / GATM","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"84053a83-8fcc-5068-8df9-58f7e97fad76","slug":"guanidinoacetate","display_name":"Guanidinoacetate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/9218780.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"407baedc2f982a5abb9d14a01e8ac9dc331d60115acc716604c1e6003aa12c1b\", \"start_char\": 0, \"end_char\": 1112, \"text_sha256\": \"407baedc2f982a5abb9d14a01e8ac9dc331d60115acc716604c1e6003aa12c1b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human AGAT crystal structures and inactive-mutant substrate complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Native, ornithine-bound and inactive mutant structures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structures support an amidino-transfer mechanism; substrate availability in a person and effects of amino-acid supplementation were not measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Recombinant human enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The first synthesis step joins material from arginine and glycine to make the precursor of creatine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p9218780] Crystal structure and mechanism of human L-arginine:glycine amidinotransferase: a mitochondrial enzyme involved in creatine biosynthesis. 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(1997). https://pubmed.ncbi.nlm.nih.gov/9218780/ DOI: 10.1093/emboj/16.12.3373","model_system":"Human AGAT crystal structures and inactive-mutant substrate complex","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [creatine-p9218780] Crystal structure and mechanism of human L-arginine:glycine amidinotransferase: a mitochondrial enzyme involved in creatine biosynthesis. 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