{"id":"8e4dcf14-23ff-57fa-a40a-653ac98bd55f","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-residual-catalytic-defect","predicate":"does-not-normalize","statement":"FAD-saturated p.Ser495del and p.Arg530Cys FADS2 remained substantially less catalytically active than wild type.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8cb61a31-865a-51d2-ab0c-d0d342eb0de8","mechanism_event_label":"Stabilizing a variant does not necessarily restore normal enzyme output.","subject":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"object":{"id":"2aba3554-0231-532b-84df-8d31f0d25846","slug":"fad-synthesis","display_name":"FAD synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8cb61a31-865a-51d2-ab0c-d0d342eb0de8","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-residual-catalytic-defect-event","event_type":"biochemical_relationship","label":"Stabilizing a variant does not necessarily restore normal enzyme output.","description":"FAD-saturated p.Ser495del and p.Arg530Cys FADS2 remained substantially less catalytically active than wild type.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"reconstitution-ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"043a308c-1d5b-5003-8c9f-6e1acd588ac3","slug":"flad1-s495del","display_name":"FLAD1 p.Ser495del variant","entity_type_key":"protein_state"},"role":"variant-enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"beaf29ae-7ea5-5004-9a0f-94f7537c1122","slug":"flad1-r530c","display_name":"FLAD1 p.Arg530Cys variant","entity_type_key":"protein_state"},"role":"variant-enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2aba3554-0231-532b-84df-8d31f0d25846","slug":"fad-synthesis","display_name":"FAD synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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