{"id":"8e4469b1-89da-52f5-92a8-7d2a1f04a7b9","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nmda-block","predicate":"blocks","statement":"Extracellular agmatine blocked NMDA currents in rat hippocampal neurons in a voltage- and concentration-dependent manner; estimated pore-site Kd was 952 micromolar at 0 mV.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e4dcb035-bae7-5efd-b45d-918ed9aa4bfc","mechanism_event_label":"Its glutamate-receptor effect depends on electrical conditions and concentration.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"e4dcb035-bae7-5efd-b45d-918ed9aa4bfc","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nmda-block-event","event_type":"observed_relationship","label":"Its glutamate-receptor effect depends on electrical conditions and concentration.","description":"Extracellular agmatine blocked NMDA currents in rat hippocampal neurons in a voltage- and concentration-dependent manner; 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whole-cell patch clamp.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The inferred pore site is not a solved binding structure or measured oral human brain exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Its glutamate-receptor effect depends on electrical conditions and concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine selectively blocks the N-methyl-D-aspartate subclass of glutamate receptor channels in rat hippocampal neurons. · 1999 · https://pubmed.ncbi.nlm.nih.gov/9918557/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2139eb5f-b588-5f6e-89fc-ca8650c17706","evidence_kind":"source_excerpt","locator":"Lines 260-266","start_line":260,"end_line":266,"excerpt":"## agmatine-sulfate-nmda-block\nIts glutamate-receptor effect depends on electrical conditions and concentration.\nExtracellular agmatine blocked NMDA currents in rat hippocampal neurons in a voltage- and concentration-dependent manner; estimated pore-site Kd was 952 micromolar at 0 mV.\nModel: Cultured rat hippocampal neurons; whole-cell patch clamp.\nLimitations: The inferred pore site is not a solved binding structure or measured oral human brain exposure.\nEvidence access: Primary abstract\nAgmatine selectively blocks the N-methyl-D-aspartate subclass of glutamate receptor channels in rat hippocampal neurons. · 1999 · https://pubmed.ncbi.nlm.nih.gov/9918557/","model_system":"Cultured rat hippocampal neurons; whole-cell patch clamp.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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