{"id":"8e39edd4-9618-5b37-b872-00b471d455d3","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:c-reg-jhdm-reprogramming-effectors","predicate":"loss-of-function-reduces-vitamin-c-supported","statement":"Jhdm1a/1b gain- and loss-of-function experiments identified these histone demethylases as functional effectors of vitamin-C-enhanced mouse somatic-cell reprogramming. Loss-of-function reduced the response rather than being corrected by vitamin C alone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"727536f5-6863-5dc6-af78-f3deda66e11c","mechanism_event_label":"The histone enzymes were part of the machinery through which vitamin C helped cells reprogram.","subject":{"id":"b25f7ed3-d218-537f-b54c-0aa9a0f9f3ab","slug":"kdm2a-mouse-gene","display_name":"Mouse Kdm2a gene","entity_type_key":"gene"},"object":{"id":"5ca7839a-4c87-5173-a476-efddb22578d2","slug":"somatic-cell-reprogramming","display_name":"Reprogramming to induced pluripotency","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"727536f5-6863-5dc6-af78-f3deda66e11c","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:c-reg-jhdm-reprogramming-effectors-event","event_type":"biochemical_relationship","label":"The histone enzymes were part of the machinery through which vitamin C helped cells reprogram.","description":"Jhdm1a/1b gain- and loss-of-function experiments identified these histone demethylases as functional effectors of vitamin-C-enhanced mouse somatic-cell reprogramming. Loss-of-function reduced the response rather than being corrected by vitamin C alone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"da1a7137-08f2-5a36-8bb6-377fbd32e327","slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"},"role":"nutrient context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1fef0b19-2e17-5701-9783-136414b12d38","slug":"kdm2a-mouse","display_name":"Mouse Kdm2a / Jhdm1a","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fba55769-52da-5b79-a90b-c469781d5d46","slug":"kdm2b-mouse","display_name":"Mouse Kdm2b / Jhdm1b","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eb125df8-33c5-5e18-a7bc-f68ccba3b838","slug":"kdm2b-mouse-gene","display_name":"Mouse Kdm2b gene","entity_type_key":"gene"},"role":"jointly investigated loss-of-function gene","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b25f7ed3-d218-537f-b54c-0aa9a0f9f3ab","slug":"kdm2a-mouse-gene","display_name":"Mouse Kdm2a gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"5ca7839a-4c87-5173-a476-efddb22578d2","slug":"somatic-cell-reprogramming","display_name":"Reprogramming to induced pluripotency","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract gain/loss-of-function result","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse fibroblast reprogramming with Jhdm1a/1b gain/loss of function","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Genetic gain/loss interventions during vitamin C-supported reprogramming; exact construct, dose and timing not recovered.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract-level mechanistic assignment; no quantitative effect size or specific knockdown efficiency asserted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The histone enzymes were part of the machinery through which vitamin C helped cells reprogram.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[c-reg-wang] The histone demethylases Jhdm1a/1b enhance somatic cell reprogramming in a vitamin-C-dependent manner. (2011). https://pubmed.ncbi.nlm.nih.gov/22100412/ DOI: 10.1016/j.stem.2011.10.005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryonic fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ffd4ec9c-b436-5203-9852-c1553dc4c581","evidence_kind":"source_excerpt","locator":"Lines 1103-1115","start_line":1103,"end_line":1115,"excerpt":"### c-reg-jhdm-reprogramming-effectors\nJhdm1a/1b gain- and loss-of-function experiments identified these histone demethylases as functional effectors of vitamin-C-enhanced mouse somatic-cell reprogramming. Loss-of-function reduced the response rather than being corrected by vitamin C alone.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The histone enzymes were part of the machinery through which vitamin C helped cells reprogram.\norganism: Mus musculus\ntissue_or_cell_type: Embryonic fibroblasts\nexperimental_model: Mouse fibroblast reprogramming with Jhdm1a/1b gain/loss of function\nlimitations: Abstract-level mechanistic assignment; no quantitative effect size or specific knockdown efficiency asserted.\nexposure: Genetic gain/loss interventions during vitamin C-supported reprogramming; exact construct, dose and timing not recovered.\ncross_nutrient: false\nevidence_location: Primary abstract gain/loss-of-function result\n[c-reg-wang] The histone demethylases Jhdm1a/1b enhance somatic cell reprogramming in a vitamin-C-dependent manner. (2011). https://pubmed.ncbi.nlm.nih.gov/22100412/ DOI: 10.1016/j.stem.2011.10.005","model_system":"Mouse fibroblast reprogramming with Jhdm1a/1b gain/loss of function","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [c-reg-wang] The histone demethylases Jhdm1a/1b enhance somatic cell reprogramming in a vitamin-C-dependent manner. (2011). https://pubmed.ncbi.nlm.nih.gov/22100412/ DOI: 10.1016/j.stem.2011.10.005","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b7fd83f956abb81855f2ea23199ba14e465cd91f4a2ac560277ec21ddafd7bfc","revision_id":"6cde9bbb-d712-5746-82a6-85b144253efa","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}