{"id":"8e268087-df92-56ec-b0fb-5c148cf1c247","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-s38-formate-rescue","predicate":"rescues_tested","statement":"Formate at 1 mM rescued the low-B6 proliferation defect of SLC25A38-null K562 cells, while glycine, serine and folate did not under the tested conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"936d2ac0-5548-5c80-b73a-b1617a164736","mechanism_event_label":"Restoring a downstream one-carbon source worked where adding an amino acid did not.","subject":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"object":{"id":"38b971ec-cfec-5a0c-8747-7b75ff6586db","slug":"human-k562-slc25a38-growth","display_name":"Growth of SLC25A38-null human K562 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"936d2ac0-5548-5c80-b73a-b1617a164736","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-s38-formate-rescue-event","event_type":"observed_relationship","label":"Restoring a downstream one-carbon source worked where adding an amino acid did not.","description":"Formate at 1 mM rescued the low-B6 proliferation defect of SLC25A38-null K562 cells, while glycine, serine and folate did not under the tested conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38b971ec-cfec-5a0c-8747-7b75ff6586db","slug":"human-k562-slc25a38-growth","display_name":"Growth of SLC25A38-null human K562 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4e146c00-9b97-55d6-ac1c-0a0261378be3","slug":"slc25a38","display_name":"Human SLC25A38","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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