{"id":"8e2383b8-cbc3-5290-b3d0-56303826603a","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-inhibits-resistant-hbv-strains","predicate":"reduces","statement":"(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"bd1698f4-8fb1-5f98-973e-7636af0fb12c","mechanism_event_label":"(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.","subject":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"object":{"id":"cfd540e7-3663-5a86-9671-e083bb84e841","slug":"hbv-dna-replication","display_name":"Hepatitis B virus DNA replication","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bd1698f4-8fb1-5f98-973e-7636af0fb12c","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-inhibits-resistant-hbv-strains-event","event_type":"observed_relationship","label":"(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.","description":"(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cfd540e7-3663-5a86-9671-e083bb84e841","slug":"hbv-dna-replication","display_name":"Hepatitis B virus DNA replication","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b6b0e047-df6c-5f77-a751-51019c928484","slug":"hbv-rna-production","display_name":"Hepatitis B virus RNA production","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hepatoma cells carrying wild-type and nucleos(t)ide-analogue-resistant hepatitis B virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"(−)-Lariciresinol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity against resistant strains is consistent with a host target rather than the viral polymerase; the abstract does not itself demonstrate the host target is necessary.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Hepatoma cells carrying wild-type and nucleos(t)ide-analogue-resistant hepatitis B virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Viral DNA replication","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cd376c1d-695a-5861-a579-11ab9f4da147","evidence_kind":"source_excerpt","locator":"Lines 99-108","start_line":99,"end_line":108,"excerpt":"## minus-lariciresinol-inhibits-resistant-hbv-strains\n(−)-Lariciresinol inhibited hepatitis B virus DNA replication of both wild-type and nucleoside analogue resistant strains.\nModel/species: Hepatoma cells carrying wild-type and nucleos(t)ide-analogue-resistant hepatitis B virus\nTissue/system: Viral DNA replication\nExposure: (−)-Lariciresinol\nRoute: In vitro\nDuration: Not stated here\nLimits: Activity against resistant strains is consistent with a host target rather than the viral polymerase; the abstract does not itself demonstrate the host target is necessary.\nPrimary reference: Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}