{"id":"8e1b74ec-279a-5cfc-b2ea-7024b9f2d392","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-complex-i-tail","predicate":"supports_rapid_exchange_in","statement":"Ubiquinone-10 had the highest binding affinity and fastest binding rate among the tested chain lengths; ubiquinol-10 release was not rate limiting.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"de30b5d4-bc9c-503e-93e1-e15cc34681d0","mechanism_event_label":"The ten-unit tail is part of the working molecule, not an inert label.","subject":{"id":"64f0151b-6c0d-5adf-a6b4-13056bc8dfb3","slug":"ubiquinone-10","display_name":"Ubiquinone-10","entity_type_key":"small_molecule"},"object":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"de30b5d4-bc9c-503e-93e1-e15cc34681d0","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-complex-i-tail-event","event_type":"biochemical_relationship","label":"The ten-unit tail is part of the working molecule, not an inert label.","description":"Ubiquinone-10 had the highest binding affinity and fastest binding rate among the tested chain lengths; ubiquinol-10 release was not rate limiting.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64f0151b-6c0d-5adf-a6b4-13056bc8dfb3","slug":"ubiquinone-10","display_name":"Ubiquinone-10","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/29133414.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\", \"start_char\": 0, \"end_char\": 1770, \"text_sha256\": \"139122e8a567596e6ec1b4bd361567d0e5cd4e3329fec72d47b949a86d29610d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Proteoliposome enzyme kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ubiquinones with one to ten isoprenoid units","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified enzyme system; short-chain analogues do not have identical binding and release kinetics to Q10.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mammalian complex I preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The ten-unit tail is part of the working molecule, not an inert label.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p29133414] Correlating kinetic and structural data on ubiquinone binding and reduction by respiratory complex I. 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