{"id":"8dafa28d-c1f7-58a5-9c70-261b15ae1314","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-collagen-transcript-null","predicate":"does_not_increase","statement":"Type I collagen mRNA did not increase despite greater collagen synthesis in treated MG-63 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7894fa8f-aa88-59f6-bcd0-dad26cfba0db","mechanism_event_label":"The measured effect was not simply more collagen-gene transcription.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"a036a1dc-5877-504b-8b93-0d6e7aa48f2e","slug":"human-mg63-col1-transcription","display_name":"Type I collagen mRNA in human MG-63 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7894fa8f-aa88-59f6-bcd0-dad26cfba0db","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-collagen-transcript-null-event","event_type":"observed_relationship","label":"The measured effect was not simply more collagen-gene transcription.","description":"Type I collagen mRNA did not increase despite greater collagen synthesis in treated MG-63 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a036a1dc-5877-504b-8b93-0d6e7aa48f2e","slug":"human-mg63-col1-transcription","display_name":"Type I collagen mRNA in human MG-63 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"091a1ec0-5867-5659-b199-b73a1ac8b9c6","slug":"human-type-i-collagen","display_name":"Human type I collagen","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human osteosarcoma-derived MG-63 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Translation, processing and turnover were not all separately resolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The measured effect was not simply more collagen-gene transcription.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12633784/ · DOI 10.1016/s8756-3282(02)00950-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b4448882-444f-536b-8754-d34527b5a435","evidence_kind":"source_excerpt","locator":"Lines 128-134","start_line":128,"end_line":134,"excerpt":"## silica-collagen-transcript-null\nThe measured effect was not simply more collagen-gene transcription.\nType I collagen mRNA did not increase despite greater collagen synthesis in treated MG-63 cells.\nModel: Human osteosarcoma-derived MG-63 cells.\nLimitations: Translation, processing and turnover were not all separately resolved.\nEvidence access: Primary abstract\nOrthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12633784/ · DOI 10.1016/s8756-3282(02)00950-x","model_system":"Human osteosarcoma-derived MG-63 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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