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(2011). https://pubmed.ncbi.nlm.nih.gov/21169551/ DOI: 10.4049/jimmunol.1002342","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neutrophil-like leukemia-derived cell culture","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e656676-a186-5357-a6d9-dd1f30881afd","evidence_kind":"source_excerpt","locator":"Lines 907-918","start_line":907,"end_line":918,"excerpt":"### e-sig-delta-lox-translocation\nDelta-tocopherol pretreatment largely prevented A23187-induced redistribution of 5-LOX from cytosolic to nuclear fractions in differentiated HL-60 cells.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Delta-tocopherol kept much of the enzyme from moving to the cell location associated with leukotriene production.\norganism: Homo sapiens\ntissue_or_cell_type: Neutrophil-like leukemia-derived cell culture\nexperimental_model: DMSO-differentiated human HL-60 neutrophil-like cells; fractionation and immunoblot\nlimitations: Fractionation is a localization assay, not proof of direct enzyme inhibition; purified 5-LOX was unaffected by tocopherols at 50 µM in this paper.\nexposure: 50 µM delta-tocopherol for 10 min followed by 2.5 µM A23187 for 15 min.\ncross_nutrient: false\n[jiang2011] Natural forms of vitamin E and 13'-carboxychromanol, a long-chain vitamin E metabolite, inhibit leukotriene generation from stimulated neutrophils by blocking calcium influx and suppressing 5-lipoxygenase activity, respectively. 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