{"id":"8d4a6e79-f725-51db-9737-7d79f66020cb","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:nbce1a-loss-blunts-k-ammonia-response","predicate":"deletion-blunts","statement":"NBCe1-A deletion blunted the urinary ammonia-excretion response to potassium-free feeding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"abd33a38-54d3-5a4f-aeb8-13a82c42cd88","mechanism_event_label":"A sodium-bicarbonate transporter was needed for the full kidney ammonia response to potassium deprivation.","subject":{"id":"0c3570c2-1a53-57ec-8e62-660be066da31","slug":"slc4a4-nbce1-a","display_name":"NBCe1-A splice variant","entity_type_key":"protein"},"object":{"id":"9d4a1c5b-de06-53b1-8ee9-42f29ce4a8e4","slug":"urinary-ammonia-excretion","display_name":"Urinary total ammonia excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"abd33a38-54d3-5a4f-aeb8-13a82c42cd88","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:nbce1a-loss-blunts-k-ammonia-response-event","event_type":"biochemical_relationship","label":"A sodium-bicarbonate transporter was needed for the full kidney ammonia response to potassium deprivation.","description":"NBCe1-A deletion blunted the urinary ammonia-excretion response to potassium-free feeding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0c3570c2-1a53-57ec-8e62-660be066da31","slug":"slc4a4-nbce1-a","display_name":"NBCe1-A splice variant","entity_type_key":"protein"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9d4a1c5b-de06-53b1-8ee9-42f29ce4a8e4","slug":"urinary-ammonia-excretion","display_name":"Urinary total ammonia excretion","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"measured-pool-component","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e923077b-9786-5ab9-b23b-638777ac6a85","slug":"ammonium-ion","display_name":"Ammonium ion","entity_type_key":"ion"},"role":"measured-pool-component","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransported-ion","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"edf44f6b-7b23-5b97-a86b-c940e5812b06","slug":"bicarbonate-ion","display_name":"Bicarbonate ion","entity_type_key":"ion"},"role":"transported-ion","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Sodium-bicarbonate transport machinery is required for the full potassium-responsive ammonia adaptation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"NBCe1-A deletion blunted the urinary ammonia-excretion response to potassium-free feeding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"NBCe1-A-selective knockout mice and wild-type littermates on control or potassium-free diets; four-day response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"NBCe1-A-selective knockout mice and wild-type littermates on control or potassium-free diets; four-day response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay reports total ammonia. Knockout also causes acidosis and outer-medullary compensation; the exact signal is unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A sodium-bicarbonate transporter was needed for the full kidney ammonia response to potassium deprivation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lee-2020-nbce1a] NBCe1-A is required for the renal ammonia and K+ response to hypokalemia (2020). https://pubmed.ncbi.nlm.nih.gov/31841393/ DOI: 10.1152/ajprenal.00481.2019","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"cortical proximal tubule and urinary ammonia output","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2790fe86-57bd-5912-9d60-218fd1b0290e","evidence_kind":"source_excerpt","locator":"Lines 1082-1094","start_line":1082,"end_line":1094,"excerpt":"### nbce1a-loss-blunts-k-ammonia-response\nNBCe1-A deletion blunted the urinary ammonia-excretion response to potassium-free feeding.\nCondition category: machinery_impairment\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A sodium-bicarbonate transporter was needed for the full kidney ammonia response to potassium deprivation.\norganism: Mus musculus\ntissue_or_cell_type: cortical proximal tubule and urinary ammonia output\nexperimental_model: NBCe1-A-selective knockout mice and wild-type littermates on control or potassium-free diets; four-day response.\nlimitations: Assay reports total ammonia. Knockout also causes acidosis and outer-medullary compensation; the exact signal is unresolved.\ncross_nutrient: Sodium-bicarbonate transport machinery is required for the full potassium-responsive ammonia adaptation.\nexperimental-exposure: NBCe1-A-selective knockout mice and wild-type littermates on control or potassium-free diets; four-day response.\nendpoint: NBCe1-A deletion blunted the urinary ammonia-excretion response to potassium-free feeding.\n[lee-2020-nbce1a] NBCe1-A is required for the renal ammonia and K+ response to hypokalemia (2020). https://pubmed.ncbi.nlm.nih.gov/31841393/ DOI: 10.1152/ajprenal.00481.2019","model_system":"NBCe1-A-selective knockout mice and wild-type littermates on control or potassium-free diets; four-day response.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lee-2020-nbce1a] NBCe1-A is required for the renal ammonia and K+ response to hypokalemia (2020). https://pubmed.ncbi.nlm.nih.gov/31841393/ DOI: 10.1152/ajprenal.00481.2019","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}