{"id":"8d344ff7-c56b-598b-a6e5-07e2fd3500be","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-ftcd-formimino-transfer","predicate":"transfers-formimino-from","statement":"The FT activity of rat FTCD transfers the FIGLU formimino group to THF, yielding 5-formimino-THF and glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ad19b2b1-22d9-59c4-b2a5-9f07827e2b00","mechanism_event_label":"This step brings histidine-derived carbon into folate chemistry.","subject":{"id":"061381b6-694e-5039-a62a-3f1b8cfc9381","slug":"rat-ftcd","display_name":"Rat formiminotransferase cyclodeaminase / FTCD","entity_type_key":"protein"},"object":{"id":"39ab812e-ba38-57d7-9973-5cc8814eaa5e","slug":"figlu","display_name":"N-Formimino-L-glutamate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ad19b2b1-22d9-59c4-b2a5-9f07827e2b00","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-ftcd-formimino-transfer-event","event_type":"biochemical_relationship","label":"This step brings histidine-derived carbon into folate chemistry.","description":"The FT activity of rat FTCD transfers the FIGLU formimino group to THF, yielding 5-formimino-THF and glutamate.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0770ecec-23d8-5681-8c71-c7899075d20e","slug":"5-formiminotetrahydrofolate","display_name":"5-Formiminotetrahydrofolate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"coproduct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"upstream source of FIGLU","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"061381b6-694e-5039-a62a-3f1b8cfc9381","slug":"rat-ftcd","display_name":"Rat formiminotransferase cyclodeaminase / FTCD","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"39ab812e-ba38-57d7-9973-5cc8814eaa5e","slug":"figlu","display_name":"N-Formimino-L-glutamate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Histidine degradation supplies a folate-bound carbon group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Introduction reaction definition; Figure 1 and FT-domain structural analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant protein structure and functional analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Recombinant rat FTCD; FT-domain structural analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat enzyme; no human histidine-loading response is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This step brings histidine-derived carbon into folate chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mao-2004] Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer (2004). https://pubmed.ncbi.nlm.nih.gov/15272307/ DOI: 10.1038/sj.emboj.7600327","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4a450b51-fc09-586c-89f9-d5a2daab3806","evidence_kind":"source_excerpt","locator":"Lines 1294-1306","start_line":1294,"end_line":1306,"excerpt":"### rat-ftcd-formimino-transfer\nThe FT activity of rat FTCD transfers the FIGLU formimino group to THF, yielding 5-formimino-THF and glutamate.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This step brings histidine-derived carbon into folate chemistry.\norganism: Rattus norvegicus\ntissue_or_cell_type: Cell-free\nexperimental_model: Recombinant protein structure and functional analysis\nlimitations: Rat enzyme; no human histidine-loading response is inferred.\nexposure: Recombinant rat FTCD; FT-domain structural analysis.\ncross_nutrient: Histidine degradation supplies a folate-bound carbon group.\nevidence_location: Introduction reaction definition; Figure 1 and FT-domain structural analysis.\n[mao-2004] Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer (2004). https://pubmed.ncbi.nlm.nih.gov/15272307/ DOI: 10.1038/sj.emboj.7600327","model_system":"Recombinant protein structure and functional analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mao-2004] Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer (2004). https://pubmed.ncbi.nlm.nih.gov/15272307/ DOI: 10.1038/sj.emboj.7600327","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}