{"id":"8d31e565-d880-51f9-a20c-db15931e8aaa","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs1a-targeting","predicate":"has_splice_dependent_localization","statement":"Exon 1a targeted type I MOCS1A to the mitochondrial matrix, whereas exon 1b variants remained cytosolic.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"66a9a8f7-99b0-5d7d-b8e7-8995c0c86380","mechanism_event_label":"Splicing changes where the first assembly protein goes.","subject":{"id":"33975f8e-c4ce-5122-8969-8fa35a8d009d","slug":"mocs1a","display_name":"Human molybdenum cofactor synthesis protein MOCS1A","entity_type_key":"protein"},"object":{"id":"98a46ebe-c551-58d2-8358-7b1173134093","slug":"mocs1-cpmp-synthesis-system","display_name":"Human MOCS1A-MOCS1B cPMP synthesis system","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"66a9a8f7-99b0-5d7d-b8e7-8995c0c86380","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-mocs1a-targeting-event","event_type":"biochemical_relationship","label":"Splicing changes where the first assembly protein goes.","description":"Exon 1a targeted type I MOCS1A to the mitochondrial matrix, whereas exon 1b variants remained cytosolic.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33975f8e-c4ce-5122-8969-8fa35a8d009d","slug":"mocs1a","display_name":"Human molybdenum cofactor synthesis protein MOCS1A","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"98a46ebe-c551-58d2-8358-7b1173134093","slug":"mocs1-cpmp-synthesis-system","display_name":"Human MOCS1A-MOCS1B cPMP synthesis system","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/31996372.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\", \"start_char\": 0, \"end_char\": 1846, \"text_sha256\": \"a88a2e2c4aae83293fa6f1860d10bb41b41c32d1ad79b0b04cd5dd3eb02865c7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Alternative splicing, fluorescence microscopy, fractionation and mitochondrial targeting of human MOCS1 proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Type I-III MOCS1 splice constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Localization and processing experiments; no dietary iron, SAM or molybdenum intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Splicing changes where the first assembly protein goes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p31996372] Alternative splicing of the bicistronic gene molybdenum cofactor synthesis 1 (MOCS1) uncovers a novel mitochondrial protein maturation mechanism. 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