{"id":"8d190052-3565-5dcf-9c9c-90a78f0e6dfa","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-oat1-uptake","predicate":"reported_relationship","statement":"Human OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"a6e41020-e4d9-5ba9-96e4-f3a37050156a","mechanism_event_label":"Human OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.","subject":{"id":"4872be13-147b-53b0-bd51-c717906aa430","slug":"slc22a6","display_name":"Human organic anion transporter 1 / SLC22A6","entity_type_key":"protein"},"object":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a6e41020-e4d9-5ba9-96e4-f3a37050156a","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-oat1-uptake-event","event_type":"biochemical_relationship","label":"Human OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.","description":"### ra-oat1-uptake\n\nHuman OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.\nProbenecid reduced uptake about 78%; other tested transporters did not show RA uptake above mock.\nModel and exposure: HEK293 transfectants; 10 micromolar RA uptake for 5 min, plus concentration series.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Involvement of Organic Anion Transporters in the Pharmacokinetics and Drug Interaction of Rosmarinic Acid. | 2021 | https://pubmed.ncbi.nlm.nih.gov/33435470/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC7828042/ | https://doi.org/10.3390/pharmaceutics13010083 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4872be13-147b-53b0-bd51-c717906aa430","slug":"slc22a6","display_name":"Human organic anion transporter 1 / SLC22A6","entity_type_key":"protein"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK293 transfectants; 10 micromolar RA uptake for 5 min, plus concentration series.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Involvement of Organic Anion Transporters in the Pharmacokinetics and Drug Interaction of Rosmarinic Acid. | https://pubmed.ncbi.nlm.nih.gov/33435470/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC7828042/ | https://doi.org/10.3390/pharmaceutics13010083","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-oat1-uptake; Probenecid reduced uptake about 78%; other tested transporters did not show RA uptake above mock.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2d76fc73-efc0-5704-b9f3-00c7320919a8","evidence_kind":"source_excerpt","locator":"Lines 365-371","start_line":365,"end_line":371,"excerpt":"### ra-oat1-uptake\n\nHuman OAT1 expression increased cellular RA uptake about 115-fold; measured Km was 26.5 micromolar.\nProbenecid reduced uptake about 78%; other tested transporters did not show RA uptake above mock.\nModel and exposure: HEK293 transfectants; 10 micromolar RA uptake for 5 min, plus concentration series.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Involvement of Organic Anion Transporters in the Pharmacokinetics and Drug Interaction of Rosmarinic Acid. | 2021 | https://pubmed.ncbi.nlm.nih.gov/33435470/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC7828042/ | https://doi.org/10.3390/pharmaceutics13010083 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"HEK293 transfectants; 10 micromolar RA uptake for 5 min, plus concentration series.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}