{"id":"8d149ae7-b169-56b5-8116-043dde8e9b39","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:kinase-mg","predicate":"reported_relationship","statement":"The studied Erwinia shikimate kinase requires magnesium for its ATP-dependent reaction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7fa03395-12de-592d-967f-d087ca7ffad7","mechanism_event_label":"The studied Erwinia shikimate kinase requires magnesium for its ATP-dependent reaction.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"8432bb0b-32d5-5451-8016-f630f2b8a199","slug":"erwinia-chrysanthemi-shikimate-kinase","display_name":"Erwinia chrysanthemi shikimate kinase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7fa03395-12de-592d-967f-d087ca7ffad7","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:kinase-mg-event","event_type":"biochemical_relationship","label":"The studied Erwinia shikimate kinase requires magnesium for its ATP-dependent reaction.","description":"**ATP and magnesium at shikimate kinase.** Shikimate kinase transfers a phosphoryl group from ATP to shikimate, yielding shikimate-3-phosphate and ADP. The Erwinia chrysanthemi enzyme study identifies magnesium dependence and a Mg–ADP structural complex; this is a microbial enzyme requirement, not a demonstrated human magnesium-deficiency phenotype. E. coli has AroK and AroL kinase isoenzymes with different substrate affinities. The AroK structural paper discusses approximate shikimate Km values of 20 mM versus 0.2 mM for the two systems; these are organism/assay properties, not plasma targets. Loss of both kinases produces aromatic-amino-acid auxotrophy; environmental nutrient rescue and isoenzyme compensation matter. [Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine.](https://pubmed.ncbi.nlm.nih.gov/11369852/) [Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam.](https://pubmed.ncbi.nlm.nih.gov/12001235/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"8432bb0b-32d5-5451-8016-f630f2b8a199","slug":"erwinia-chrysanthemi-shikimate-kinase","display_name":"Erwinia chrysanthemi shikimate kinase","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphoryl donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fe7a4dc3-4f06-586b-9457-2dd949e2fa36","slug":"shikimate","display_name":"Shikimate anion","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text HTML and indexed abstract reviewed for the stated results; archived locally.\nPrimary publisher article introductory text reviewed; precise affinity estimates are contextual, not newly remeasured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified Erwinia chrysanthemi enzyme and Mg–ADP structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human magnesium dose threshold or E. coli-specific quantitative affinity inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The studied Erwinia shikimate kinase requires magnesium for its ATP-dependent reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine. | 2001 | DOI 10.1110/ps.52501 | PMID 11369852 | https://pubmed.ncbi.nlm.nih.gov/11369852/ | https://doi.org/10.1110/ps.52501 | https://pmc.ncbi.nlm.nih.gov/articles/PMC2374015/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam. | 2002 | DOI 10.1002/prot.10099 | PMID 12001235 | https://pubmed.ncbi.nlm.nih.gov/12001235/ | https://doi.org/10.1002/prot.10099","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 15-15; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8eb65bda-ef1a-557a-87af-e5adb0bc3aac","evidence_kind":"source_excerpt","locator":"Lines 15-15","start_line":15,"end_line":15,"excerpt":"**ATP and magnesium at shikimate kinase.** Shikimate kinase transfers a phosphoryl group from ATP to shikimate, yielding shikimate-3-phosphate and ADP. The Erwinia chrysanthemi enzyme study identifies magnesium dependence and a Mg–ADP structural complex; this is a microbial enzyme requirement, not a demonstrated human magnesium-deficiency phenotype. E. coli has AroK and AroL kinase isoenzymes with different substrate affinities. The AroK structural paper discusses approximate shikimate Km values of 20 mM versus 0.2 mM for the two systems; these are organism/assay properties, not plasma targets. Loss of both kinases produces aromatic-amino-acid auxotrophy; environmental nutrient rescue and isoenzyme compensation matter. [Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine.](https://pubmed.ncbi.nlm.nih.gov/11369852/) [Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam.](https://pubmed.ncbi.nlm.nih.gov/12001235/)","model_system":"Purified Erwinia chrysanthemi enzyme and Mg–ADP structure.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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