{"id":"8d079cd4-be95-5d40-93f5-57b8a85bd61f","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-camk","predicate":"attenuates","statement":"CaMKII inhibitor KN-62 attenuated CLA-induced stress/inflammatory responses and loss of insulin-stimulated glucose uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"24359b5c-60d3-582f-98a9-2c89f3faa89c","mechanism_event_label":"Blocking a calcium-responsive kinase pathway weakened the response.","subject":{"id":"55332bcc-4add-5e30-9639-3c1058812e7e","slug":"kn-62","display_name":"KN-62, pharmacological CaMKII inhibitor","entity_type_key":"small_molecule"},"object":{"id":"364992bb-f7d7-5e4d-967b-b6646faafd2b","slug":"human-adipocyte-cla-inflammatory-output","display_name":"Human adipocyte CLA-associated cytokine output","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"24359b5c-60d3-582f-98a9-2c89f3faa89c","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-camk-event","event_type":"observed_relationship","label":"Blocking a calcium-responsive kinase pathway weakened the response.","description":"CaMKII inhibitor KN-62 attenuated CLA-induced stress/inflammatory responses and loss of insulin-stimulated glucose uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"55332bcc-4add-5e30-9639-3c1058812e7e","slug":"kn-62","display_name":"KN-62, pharmacological CaMKII inhibitor","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"364992bb-f7d7-5e4d-967b-b6646faafd2b","slug":"human-adipocyte-cla-inflammatory-output","display_name":"Human adipocyte CLA-associated cytokine output","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"23af756f-e575-5d73-a1fe-4334792f7bbb","slug":"camk2b","display_name":"Human calcium/calmodulin-dependent protein kinase II beta / CAMK2B","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte pharmacology; CaMKII-beta induction also measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibitor data alone do not prove CAMK2B is the sole required isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Blocking a calcium-responsive kinase pathway weakened the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inflammation and insulin resistance induced by trans-10, cis-12 conjugated linoleic acid depend on intracellular calcium levels in primary cultures of human adipocytes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20154361/ · DOI 10.1194/jlr.M005447","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"72b25b77-8075-5cb7-bfa3-bcb8a9796364","evidence_kind":"source_excerpt","locator":"Lines 158-164","start_line":158,"end_line":164,"excerpt":"## cla-camk\nBlocking a calcium-responsive kinase pathway weakened the response.\nCaMKII inhibitor KN-62 attenuated CLA-induced stress/inflammatory responses and loss of insulin-stimulated glucose uptake.\nModel: Human adipocyte pharmacology; CaMKII-beta induction also measured.\nLimitations: Inhibitor data alone do not prove CAMK2B is the sole required isoform.\nEvidence access: Primary abstract\nInflammation and insulin resistance induced by trans-10, cis-12 conjugated linoleic acid depend on intracellular calcium levels in primary cultures of human adipocytes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20154361/ · DOI 10.1194/jlr.M005447","model_system":"Human adipocyte pharmacology; CaMKII-beta induction also measured.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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