{"id":"8ca99cd3-83eb-540f-8a1d-4bb8f2dbca35","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-lat1-transport","predicate":"transports","statement":"Human LAT1 with its SLC3A2 partner transported radiolabeled methionine with an apparent Km of 99 +/- 9 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"83018836-c3a5-58f7-823d-5f9462eb7fb1","mechanism_event_label":"A shared amino-acid carrier admits methionine.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"83018836-c3a5-58f7-823d-5f9462eb7fb1","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-lat1-transport-event","event_type":"observed_relationship","label":"A shared amino-acid carrier admits methionine.","description":"Human LAT1 with its SLC3A2 partner transported radiolabeled methionine with an apparent Km of 99 +/- 9 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1ca4b4d5-616a-54d3-8244-0dc151b1bcfe","slug":"slc3a2","display_name":"4F2hc / SLC3A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human LAT1/SLC3A2 expressed in Xenopus oocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A transporter assay is not a human intestinal absorption threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A shared amino-acid carrier admits methionine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12117417/ · DOI 10.1042/BJ20020841","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Radiolabelled methionine uptake with an apparent Km of 99 micromolar.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Radiolabelled methionine uptake with an apparent Km of 99 micromolar.","entity":null}],"evidence":[{"id":"e3aebfe9-482d-51e9-94d6-159469309d1e","evidence_kind":"source_excerpt","locator":"Lines 20-26","start_line":20,"end_line":26,"excerpt":"## methionine-lat1-transport\nA shared amino-acid carrier admits methionine.\nHuman LAT1 with its SLC3A2 partner transported radiolabeled methionine with an apparent Km of 99 +/- 9 micromolar.\nModel: Human LAT1/SLC3A2 expressed in Xenopus oocytes.\nLimitations: A transporter assay is not a human intestinal absorption threshold.\nEvidence access: Primary abstract\nTransport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12117417/ · DOI 10.1042/BJ20020841","model_system":"Human LAT1/SLC3A2 expressed in Xenopus oocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f1c706ef72932eed85df234b6c3304158d503d626a63c69b02a453c162700d26","revision_id":"549035e0-3a72-550a-8a55-e8c5baa51b0d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}