{"id":"8c8a1835-2707-5a77-b7f5-43adac6cd23d","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-hdl-delivery","predicate":"increases","statement":"HDL increased zeaxanthin uptake mediated by the tested SRB proteins.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f0565134-9408-5b45-ae67-9680070d62f8","mechanism_event_label":"The carrier and receptor worked together in this model.","subject":{"id":"5777d3b5-1e4a-5a21-a6c4-0a3c2754a8b6","slug":"hdl-particles","display_name":"High-density lipoprotein particles; preparation specified","entity_type_key":"chemical_species"},"object":{"id":"bbf2bee1-db69-5061-8f6a-22d9db34c1b1","slug":"hek293-zeaxanthin-uptake","display_name":"Zeaxanthin uptake in SRB-transfected cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f0565134-9408-5b45-ae67-9680070d62f8","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-hdl-delivery-event","event_type":"biochemical_relationship","label":"The carrier and receptor worked together in this model.","description":"HDL increased zeaxanthin uptake mediated by the tested SRB proteins.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a60c685-7502-5ece-9da2-2c1a2e3bcdf1","slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5777d3b5-1e4a-5a21-a6c4-0a3c2754a8b6","slug":"hdl-particles","display_name":"High-density lipoprotein particles; preparation specified","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bbf2bee1-db69-5061-8f6a-22d9db34c1b1","slug":"hek293-zeaxanthin-uptake","display_name":"Zeaxanthin uptake in SRB-transfected cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zeaxanthin-research/28947101.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8899547af674d9bc7acc413d58c440726a1469ca705aa82bc5dafd5c89aadcc2\", \"start_char\": 0, \"end_char\": 1330, \"text_sha256\": \"8899547af674d9bc7acc413d58c440726a1469ca705aa82bc5dafd5c89aadcc2\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"SPR and transporter overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SR-BI, SCARB2/LIMP-II and CD36; HDL or LDL carriers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The study used SCARB2/LIMP-II (methods reagent AF1966), not the similarly named SCARB1 splice variant SR-BII. Reagent identity: https://www.rndsystems.com/products/human-limpii-sr-b2-antibody_af1966 . Transporter overexpression is not an oral absorption trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zeaxanthin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins in HEK-293T cells and donor eye tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The carrier and receptor worked together in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zeaxanthin-p28947101] All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids. (2017). https://pubmed.ncbi.nlm.nih.gov/28947101/ DOI: 10.1016/j.abb.2017.09.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant receptors and HEK-293T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d087129c-0d0f-553d-a2ad-2be51dcfc618","evidence_kind":"source_excerpt","locator":"Lines 626-637","start_line":626,"end_line":637,"excerpt":"### zeaxanthin-hdl-delivery\nHDL increased zeaxanthin uptake mediated by the tested SRB proteins.\nCondition category: normal\nnutrient_topic: Zeaxanthin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The carrier and receptor worked together in this model.\norganism: Human proteins in HEK-293T cells and donor eye tissue\ntissue_or_cell_type: Recombinant receptors and HEK-293T cells\nexperimental_model: SPR and transporter overexpression\nlimitations: The study used SCARB2/LIMP-II (methods reagent AF1966), not the similarly named SCARB1 splice variant SR-BII. Reagent identity: https://www.rndsystems.com/products/human-limpii-sr-b2-antibody_af1966 . Transporter overexpression is not an oral absorption trial.\nexposure: SR-BI, SCARB2/LIMP-II and CD36; HDL or LDL carriers\nevidence_span: {\"source_cache\": \"artifacts/zeaxanthin-research/28947101.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8899547af674d9bc7acc413d58c440726a1469ca705aa82bc5dafd5c89aadcc2\", \"start_char\": 0, \"end_char\": 1330, \"text_sha256\": \"8899547af674d9bc7acc413d58c440726a1469ca705aa82bc5dafd5c89aadcc2\"}\n[zeaxanthin-p28947101] All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids. (2017). https://pubmed.ncbi.nlm.nih.gov/28947101/ DOI: 10.1016/j.abb.2017.09.013","model_system":"SPR and transporter overexpression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zeaxanthin-p28947101] All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids. (2017). https://pubmed.ncbi.nlm.nih.gov/28947101/ DOI: 10.1016/j.abb.2017.09.013","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"df7effe5-d60c-525e-bd48-1518e1a03c51","stable_key":"import-d8050016-13c9-5391-9d61-63de73590053","title":"Zeaxanthin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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