{"id":"8c72d10a-12f3-542d-a800-b936dcd14412","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-gpx-activity","predicate":"increases","statement":"Astaxanthin increased measured glutathione-peroxidase activity in the HUVEC experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9fcb9ebf-31b4-5065-b7f3-7bc766f8bf07","mechanism_event_label":"The antioxidant enzyme response changed as well.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"d563f6a8-beb6-557d-a13e-144b6091fca9","slug":"human-gpx-activity-family","display_name":"Human glutathione peroxidase activity, isoform unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"9fcb9ebf-31b4-5065-b7f3-7bc766f8bf07","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-gpx-activity-event","event_type":"observed_relationship","label":"The antioxidant enzyme response changed as well.","description":"Astaxanthin increased measured glutathione-peroxidase activity in the HUVEC experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d563f6a8-beb6-557d-a13e-144b6091fca9","slug":"human-gpx-activity-family","display_name":"Human glutathione peroxidase activity, isoform unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human endothelial-cell enzyme-activity assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The assay does not resolve the GPX isoform, demonstrate selenium repletion, or quantify glutathione synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The antioxidant enzyme response changed as well.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Astaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6a5b3f45-3f11-5903-b127-d18fd46546d5","evidence_kind":"source_excerpt","locator":"Lines 238-244","start_line":238,"end_line":244,"excerpt":"## astaxanthin-gpx-activity\nThe antioxidant enzyme response changed as well.\nAstaxanthin increased measured glutathione-peroxidase activity in the HUVEC experiment.\nModel: Human endothelial-cell enzyme-activity assay.\nLimitations: The assay does not resolve the GPX isoform, demonstrate selenium repletion, or quantify glutathione synthesis.\nEvidence access: Primary abstract\nAstaxanthin Induces the Nrf2/HO-1 Antioxidant Pathway in Human Umbilical Vein Endothelial Cells by Generating Trace Amounts of ROS. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29381356/ · DOI 10.1021/acs.jafc.7b05493","model_system":"Human endothelial-cell enzyme-activity assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}