{"id":"8c01f879-6d10-5b56-b032-99d3454f550d","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-txnrd-radical","predicate":"catalyzes-reduction-of","statement":"Purified rat liver thioredoxin reductase decreased ascorbyl radical measured by electron paramagnetic resonance while consuming NADPH; DHA controls could not explain the signal.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"51ac41ba-a844-5cb6-a9e8-c98dfb3da8d2","mechanism_event_label":"The reductase can also recycle the one-electron vitamin C radical.","subject":{"id":"44c93642-76cb-5254-9edb-14a3020a5ae6","slug":"rat-txnrd1","display_name":"Rat thioredoxin reductase 1 / Txnrd1","entity_type_key":"protein"},"object":{"id":"17a4b1a5-451a-563f-8f20-2de8f4b0af8e","slug":"ascorbyl-radical","display_name":"Ascorbyl radical","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"51ac41ba-a844-5cb6-a9e8-c98dfb3da8d2","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-txnrd-radical-event","event_type":"biochemical_relationship","label":"The reductase can also recycle the one-electron vitamin C radical.","description":"Purified rat liver thioredoxin reductase decreased ascorbyl radical measured by electron paramagnetic resonance while consuming NADPH; DHA controls could not explain the signal.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"electron donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"reduced product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44c93642-76cb-5254-9edb-14a3020a5ae6","slug":"rat-txnrd1","display_name":"Rat thioredoxin reductase 1 / Txnrd1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"17a4b1a5-451a-563f-8f20-2de8f4b0af8e","slug":"ascorbyl-radical","display_name":"Ascorbyl radical","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified rat liver enzyme, dialyzed cytosol and microsomes from control/selenium-deficient rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Low-micromolar ascorbyl radical generated by ascorbate oxidase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified assay; radical and DHA reduction are distinct reactions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The reductase can also recycle the one-electron vitamin C radical.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[may1998] Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase. (1998). https://pubmed.ncbi.nlm.nih.gov/9722529/ DOI: 10.1074/jbc.273.36.23039","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified liver enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"97e94501-aa8f-58ee-b1d0-43559c53ca1e","evidence_kind":"source_excerpt","locator":"Lines 416-427","start_line":416,"end_line":427,"excerpt":"### vc-transport-txnrd-radical\nPurified rat liver thioredoxin reductase decreased ascorbyl radical measured by electron paramagnetic resonance while consuming NADPH; DHA controls could not explain the signal.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The reductase can also recycle the one-electron vitamin C radical.\norganism: Rattus norvegicus\ntissue_or_cell_type: Purified liver enzyme\nexperimental_model: Purified rat liver enzyme, dialyzed cytosol and microsomes from control/selenium-deficient rats\nlimitations: Purified assay; radical and DHA reduction are distinct reactions.\nexposure: Low-micromolar ascorbyl radical generated by ascorbate oxidase\ncross_nutrient: true\n[may1998] Reduction of the ascorbyl free radical to ascorbate by thioredoxin reductase. 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