{"id":"8bf25420-af47-521e-940f-7e25b46b2fef","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-pemt-vldl","predicate":"reduces_in_ldlr_null_background","statement":"Pemt deletion modestly reduced hepatic VLDL secretion in Ldlr-null mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6ca20993-e365-56a4-ba60-5eb664c970c5","mechanism_event_label":"PC synthesis is connected to how the liver exports lipid.","subject":{"id":"8fc612e2-a610-52e9-bb14-d4a6ad5b8ab3","slug":"mouse-pemt-null","display_name":"Experimental mouse Pemt-null genotype","entity_type_key":"protein_state"},"object":{"id":"a385ae7e-9045-5e2a-83e0-c1b2c2ff191f","slug":"mouse-hepatic-vldl-secretion","display_name":"Mouse hepatic VLDL secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6ca20993-e365-56a4-ba60-5eb664c970c5","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-pemt-vldl-event","event_type":"biochemical_relationship","label":"PC synthesis is connected to how the liver exports lipid.","description":"Pemt deletion modestly reduced hepatic VLDL secretion in Ldlr-null mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"146a29a4-a5a8-5aa6-9838-d833aec6e4cb","slug":"mouse-pemt","display_name":"Mouse phosphatidylethanolamine N-methyltransferase / Pemt","entity_type_key":"protein"},"role":"missing_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f77ef6d-3ecd-5a80-9ca3-3cbea403d9fc","slug":"phosphatidylcholine","display_name":"Phosphatidylcholine","entity_type_key":"lipid"},"role":"lipoprotein_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8fc612e2-a610-52e9-bb14-d4a6ad5b8ab3","slug":"mouse-pemt-null","display_name":"Experimental mouse Pemt-null genotype","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a385ae7e-9045-5e2a-83e0-c1b2c2ff191f","slug":"mouse-hepatic-vldl-secretion","display_name":"Mouse hepatic VLDL secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/19520976.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"57c952bdb4e387632c90b6dcc00ab5f0c600047b7e78bb179c08f6ccd62704d0\", \"start_char\": 0, \"end_char\": 1537, \"text_sha256\": \"57c952bdb4e387632c90b6dcc00ab5f0c600047b7e78bb179c08f6ccd62704d0\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pemt/Ldlr mouse knockout under a high-fat/high-cholesterol diet","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sixteen weeks of the specified diet; Pemt-null/Ldlr-null versus Pemt-intact/Ldlr-null","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A lipid-export mechanism in a specific genetic model; lower plasma lipids do not establish healthier liver tissue or dietary benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PC synthesis is connected to how the liver exports lipid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p19520976] Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. (2009). https://pubmed.ncbi.nlm.nih.gov/19520976/ DOI: 10.1161/atvbaha.109.188672","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver and circulating VLDL","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"db4a9e4b-a032-59c3-96ad-1db61e220e31","evidence_kind":"source_excerpt","locator":"Lines 932-943","start_line":932,"end_line":943,"excerpt":"### choline-pemt-vldl\nPemt deletion modestly reduced hepatic VLDL secretion in Ldlr-null mice.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PC synthesis is connected to how the liver exports lipid.\norganism: Mouse\ntissue_or_cell_type: Liver and circulating VLDL\nexperimental_model: Pemt/Ldlr mouse knockout under a high-fat/high-cholesterol diet\nlimitations: A lipid-export mechanism in a specific genetic model; lower plasma lipids do not establish healthier liver tissue or dietary benefit.\nexposure: Sixteen weeks of the specified diet; Pemt-null/Ldlr-null versus Pemt-intact/Ldlr-null\nevidence_span: {\"source_cache\": \"artifacts/choline-research/19520976.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"57c952bdb4e387632c90b6dcc00ab5f0c600047b7e78bb179c08f6ccd62704d0\", \"start_char\": 0, \"end_char\": 1537, \"text_sha256\": \"57c952bdb4e387632c90b6dcc00ab5f0c600047b7e78bb179c08f6ccd62704d0\"}\n[choline-p19520976] Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. (2009). https://pubmed.ncbi.nlm.nih.gov/19520976/ DOI: 10.1161/atvbaha.109.188672","model_system":"Pemt/Ldlr mouse knockout under a high-fat/high-cholesterol diet","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [choline-p19520976] Lack of phosphatidylethanolamine N-methyltransferase alters plasma VLDL phospholipids and attenuates atherosclerosis in mice. (2009). https://pubmed.ncbi.nlm.nih.gov/19520976/ DOI: 10.1161/atvbaha.109.188672","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0ba2f05e-5cf5-5bd7-ae5d-0ab4945fcb7b","stable_key":"import-27e0c1cf-7726-5164-8b15-27a631584cd0","title":"Choline: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c7afe3d579eba0df6d2a4c26699c653462749e03188348f900c07748cc7282a8","revision_id":"0fa7b48f-2a3c-5adc-b13e-48727dd6f815","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}