{"id":"8bd46ed4-18ec-5407-85b9-eb314fdd594f","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cyp27a1-d2-c27","predicate":"produces","statement":"Human CYP27A1 hydroxylated ergocalciferol at C27 in the reported D2 substrate analysis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ee3aa070-2c73-5bca-8ad5-1a25612ccadf","mechanism_event_label":"CYP27A1 modifies D2 at carbon 27.","subject":{"id":"a51f023f-7880-5e68-a72b-00293792ff64","slug":"cyp27a1","display_name":"Human sterol 27-hydroxylase / CYP27A1","entity_type_key":"protein"},"object":{"id":"9beda1d0-e66c-5108-84b2-575c4a8d9e44","slug":"27-hydroxyvitamin-d2","display_name":"27-Hydroxyvitamin D2","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ee3aa070-2c73-5bca-8ad5-1a25612ccadf","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cyp27a1-d2-c27-event","event_type":"biochemical_relationship","label":"CYP27A1 modifies D2 at carbon 27.","description":"Human CYP27A1 hydroxylated ergocalciferol at C27 in the reported D2 substrate analysis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a51f023f-7880-5e68-a72b-00293792ff64","slug":"cyp27a1","display_name":"Human sterol 27-hydroxylase / CYP27A1","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9beda1d0-e66c-5108-84b2-575c4a8d9e44","slug":"27-hydroxyvitamin-d2","display_name":"27-Hydroxyvitamin D2","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract, substrate product positions and kinetic comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human CYP27A1 product identification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"D2 incubation; concentration/time not reported in retrieved abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"D2 C24/C27 hydroxylation must not be mislabeled as C25 activation or assumed to be the main route in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CYP27A1 modifies D2 at carbon 27.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[shinkyo2004] Metabolism of vitamin D by human microsomal CYP2R1. (2004). https://pubmed.ncbi.nlm.nih.gov/15465040/ DOI: 10.1016/j.bbrc.2004.09.073","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"mitochondrial enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"095a50cb-dca6-5df0-9fe3-03842a4a4772","evidence_kind":"source_excerpt","locator":"Lines 347-360","start_line":347,"end_line":360,"excerpt":"### vd-act-cyp27a1-d2-c27\nHuman CYP27A1 hydroxylated ergocalciferol at C27 in the reported D2 substrate analysis.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CYP27A1 modifies D2 at carbon 27.\norganism: Homo sapiens protein\ntissue_or_cell_type: mitochondrial enzyme preparation\nexperimental_model: Recombinant human CYP27A1 product identification\nlimitations: D2 C24/C27 hydroxylation must not be mislabeled as C25 activation or assumed to be the main route in vivo.\nexposure: D2 incubation; concentration/time not reported in retrieved abstract.\ncross_nutrient: false\nevidence_location: Primary abstract, substrate product positions and kinetic comparison.\nnutrient: Vitamin D2 and D3\n[shinkyo2004] Metabolism of vitamin D by human microsomal CYP2R1. 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