{"id":"8bd23b4b-0db0-5df9-9c2e-145204cbedb4","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-pgp-atpase-null","predicate":"no_detected_stimulation","statement":"Tartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"8a9dbd38-d3b7-5c90-892e-9f5a07537ed2","mechanism_event_label":"Tartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"33203258-d37b-5d44-acca-dc40841a9761","slug":"human-abcb1-atpase-assay","display_name":"ATPase activity of recombinant human ABCB1 / P-glycoprotein","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8a9dbd38-d3b7-5c90-892e-9f5a07537ed2","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-pgp-atpase-null-event","event_type":"observed_relationship","label":"Tartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.","description":"Tartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"33203258-d37b-5d44-acca-dc40841a9761","slug":"human-abcb1-atpase-assay","display_name":"ATPase activity of recombinant human ABCB1 / P-glycoprotein","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c6139b52-4140-5622-9e33-1f6c6385265d","slug":"abcb1","display_name":"Human P-glycoprotein / ABCB1 / MDR1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 0.41-900 micromolar; verapamil and orthovanadate controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Manufacturer ATPase assay interval not stated in accessed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human P-glycoprotein in membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A negative ATPase-stimulation result is bounded by the assay and does not establish universal lack of transport interactions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Recombinant human P-glycoprotein in membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Food dyes as P-glycoprotein modulators. (2020). https://pubmed.ncbi.nlm.nih.gov/33011351/ DOI: 10.1016/j.fct.2020.111785","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro membrane assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"ATPase stimulation assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f9e92ff4-236d-550c-8031-806c4851c159","evidence_kind":"source_excerpt","locator":"Lines 226-235","start_line":226,"end_line":235,"excerpt":"## tartrazine-pgp-atpase-null\nTartrazine did not stimulate P-glycoprotein-specific ATPase activity in the membrane assay.\nModel/species: Recombinant human P-glycoprotein in membrane preparations\nTissue: ATPase stimulation assay\nExposure: Tartrazine 0.41-900 micromolar; verapamil and orthovanadate controls\nRoute: In vitro membrane assay\nDuration: Manufacturer ATPase assay interval not stated in accessed methods\nLimits: A negative ATPase-stimulation result is bounded by the assay and does not establish universal lack of transport interactions.\nPrimary reference: Food dyes as P-glycoprotein modulators. 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