{"id":"8bceed63-3bd9-5923-bd68-9c9d421773a3","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-endo-gpx4-normal-diet","predicate":"has-no-obvious-effect-on","statement":"Endothelium-specific Gpx4 deletion produced no obvious impairment of vascular homeostasis in mice maintained on a normal diet in the reported study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"4388144c-d33a-5eb8-92e6-42660ee23ecd","mechanism_event_label":"Loss of endothelial GPX4 alone did not produce an obvious vascular phenotype under the normal-diet conditions tested.","subject":{"id":"08b2e09d-0fa9-56b8-b723-801569bf3f2f","slug":"gpx4-endothelial-loss","display_name":"Endothelium-directed GPX4 loss","entity_type_key":"protein_state"},"object":{"id":"2da43fce-d01d-5594-b067-b74f8e44a330","slug":"vascular-homeostasis","display_name":"Vascular homeostasis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4388144c-d33a-5eb8-92e6-42660ee23ecd","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-endo-gpx4-normal-diet-event","event_type":"biochemical_relationship","label":"Loss of endothelial GPX4 alone did not produce an obvious vascular phenotype under the normal-diet conditions tested.","description":"Endothelium-specific Gpx4 deletion produced no obvious impairment of vascular homeostasis in mice maintained on a normal diet in the reported study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e9bb13ee-f238-51e6-9101-78ddfd11465c","slug":"gpx4","display_name":"GPX4","entity_type_key":"protein"},"role":"deleted enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"08b2e09d-0fa9-56b8-b723-801569bf3f2f","slug":"gpx4-endothelial-loss","display_name":"Endothelium-directed GPX4 loss","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2da43fce-d01d-5594-b067-b74f8e44a330","slug":"vascular-homeostasis","display_name":"Vascular homeostasis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Endothelial Gpx4 conditional deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Normal diet; no quantitative vitamin E content assigned from abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary abstract only; vascular and survival endpoints in one mouse deletion model, not general human deficiency or dosing evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of endothelial GPX4 alone did not produce an obvious vascular phenotype under the normal-diet conditions tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-wortmann2013] Combined deficiency in glutathione peroxidase 4 and vitamin E causes multiorgan thrombus formation and early death in mice. (2013). https://pubmed.ncbi.nlm.nih.gov/23770613/ DOI: 10.1161/circresaha.113.279984","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Vascular endothelium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"a46bf87c-fd19-567f-935c-eddae838d82f","evidence_kind":"source_excerpt","locator":"Lines 634-646","start_line":634,"end_line":646,"excerpt":"### ver-endo-gpx4-normal-diet\nEndothelium-specific Gpx4 deletion produced no obvious impairment of vascular homeostasis in mice maintained on a normal diet in the reported study.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of endothelial GPX4 alone did not produce an obvious vascular phenotype under the normal-diet conditions tested.\norganism: Mus musculus\ntissue_or_cell_type: Vascular endothelium\nexperimental_model: Endothelial Gpx4 conditional deletion\nlimitations: Primary abstract only; vascular and survival endpoints in one mouse deletion model, not general human deficiency or dosing evidence.\nexposure: Normal diet; no quantitative vitamin E content assigned from abstract.\ncross_nutrient: true\nevidence_location: Primary abstract\n[ver-wortmann2013] Combined deficiency in glutathione peroxidase 4 and vitamin E causes multiorgan thrombus formation and early death in mice. 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