{"id":"8b6936b1-adac-572f-8497-00eb4e3aed52","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-ascorbate-gsh-adduct-protection","predicate":"reduces","statement":"Ascorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e161296d-4d84-5f39-bea2-aec76b507ba6","mechanism_event_label":"Ascorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.","subject":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"object":{"id":"894afa83-1fa2-506e-88b7-11374cc87a7a","slug":"peroxidase-activated-eugenol-dna-adducts","display_name":"DNA adducts from peroxidase-activated eugenol","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e161296d-4d84-5f39-bea2-aec76b507ba6","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-ascorbate-gsh-adduct-protection-event","event_type":"observed_relationship","label":"Ascorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.","description":"Ascorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"894afa83-1fa2-506e-88b7-11374cc87a7a","slug":"peroxidase-activated-eugenol-dna-adducts","display_name":"DNA adducts from peroxidase-activated eugenol","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"53f51828-4ea1-5f2e-a044-e02577272436","slug":"mpo","display_name":"Human myeloperoxidase / MPO","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol with peroxidase/hydrogen peroxide plus ascorbate or glutathione","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free peroxidase systems and human HL-60 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-free protection does not prove that vitamin supplementation prevents toxicity in exposed humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Cell-free peroxidase systems and human HL-60 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Ascorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Oxidation of eugenol to form DNA adducts and 8-hydroxy-2'-deoxyguanosine: role of quinone methide derivative in DNA adduct formation. (1998). https://pubmed.ncbi.nlm.nih.gov/9525278/ DOI: 10.1093/carcin/19.3.437","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"DNA adducts and oxidative base damage","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c2e4b49-7610-56ff-b2fc-7ca6e0f5f147","evidence_kind":"source_excerpt","locator":"Lines 121-130","start_line":121,"end_line":130,"excerpt":"## eugenol-ascorbate-gsh-adduct-protection\nAscorbic acid and glutathione reduced DNA-adduct formation during in-vitro peroxidase activation of eugenol by about 66% and 90%, respectively.\nModel/species: Cell-free peroxidase systems and human HL-60 cells\nTissue/system: DNA adducts and oxidative base damage\nExposure: Eugenol with peroxidase/hydrogen peroxide plus ascorbate or glutathione\nRoute: In vitro\nDuration: Acute\nLimits: Cell-free protection does not prove that vitamin supplementation prevents toxicity in exposed humans.\nPrimary reference: Oxidation of eugenol to form DNA adducts and 8-hydroxy-2'-deoxyguanosine: role of quinone methide derivative in DNA adduct formation. (1998). https://pubmed.ncbi.nlm.nih.gov/9525278/ DOI: 10.1093/carcin/19.3.437\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Cell-free peroxidase systems and human HL-60 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}