{"id":"8b60bff8-356d-5e6f-994c-764806985435","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-iars-charging","predicate":"charges","statement":"Human IARS1 aminoacylates tRNA-Ile with isoleucine for cytosolic translation; purified-protein aminoacylation assays were used to study this reaction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2b71e12a-527b-5dd4-839a-100810384f64","mechanism_event_label":"A charging enzyme links free isoleucine to protein synthesis.","subject":{"id":"fe83b156-e71e-5193-a958-0665af0e55ae","slug":"iars1","display_name":"Human cytosolic isoleucyl-tRNA synthetase / IARS1","entity_type_key":"protein"},"object":{"id":"d7d276fe-bdbb-56f8-8c5f-e40c0851e015","slug":"human-cytosolic-isoleucyl-trna","display_name":"Human cytosolic isoleucyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"2b71e12a-527b-5dd4-839a-100810384f64","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-iars-charging-event","event_type":"observed_relationship","label":"A charging enzyme links free isoleucine to protein synthesis.","description":"Human IARS1 aminoacylates tRNA-Ile with isoleucine for cytosolic translation; purified-protein aminoacylation assays were used to study this reaction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fe83b156-e71e-5193-a958-0665af0e55ae","slug":"iars1","display_name":"Human cytosolic isoleucyl-tRNA synthetase / IARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7d276fe-bdbb-56f8-8c5f-e40c0851e015","slug":"human-cytosolic-isoleucyl-trna","display_name":"Human cytosolic isoleucyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human IARS1/tRNA assays and primary human fibroblasts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction role is distinct from the study-specific shortage substitution experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A charging enzyme links free isoleucine to protein synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Isoleucine-to-valine substitutions support cellular physiology during isoleucine deprivation. · 2025 · https://pubmed.ncbi.nlm.nih.gov/39657787/ · DOI 10.1093/nar/gkae1184","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a113dfaa-c385-5da2-b646-1bc098242367","evidence_kind":"source_excerpt","locator":"Lines 106-112","start_line":106,"end_line":112,"excerpt":"## isoleucine-iars-charging\nA charging enzyme links free isoleucine to protein synthesis.\nHuman IARS1 aminoacylates tRNA-Ile with isoleucine for cytosolic translation; purified-protein aminoacylation assays were used to study this reaction.\nModel: Human IARS1/tRNA assays and primary human fibroblasts.\nLimitations: Reaction role is distinct from the study-specific shortage substitution experiments.\nEvidence access: Primary full text\nIsoleucine-to-valine substitutions support cellular physiology during isoleucine deprivation. · 2025 · https://pubmed.ncbi.nlm.nih.gov/39657787/ · DOI 10.1093/nar/gkae1184","model_system":"Human IARS1/tRNA assays and primary human fibroblasts.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"2058ff0da379586994e7fda6ae0086c60fa3173542bfaf1fb3871ad3e09f9c6c","revision_id":"f32192d4-9913-5d35-acbc-cf90ff17995e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}