{"id":"8b594313-3c68-5905-a574-6c0a5642907a","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:gcdh-crotonyl-coa","predicate":"converted_to","statement":"FAD-containing human GCDH dehydrogenates glutaryl-CoA through glutaconyl-CoA and decarboxylates it to crotonyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc5c708f-bac2-5eb3-84e5-0eda449fdcc9","mechanism_event_label":"GCDH shortens the lysine-derived carbon chain.","subject":{"id":"d4a5cca5-73c0-59b5-a1d3-4e45d7f3e69a","slug":"glutaryl-coa","display_name":"Glutaryl-CoA","entity_type_key":"small_molecule"},"object":{"id":"c8ce07a8-dfe0-5fb9-9529-f4fe2fddc134","slug":"crotonyl-coa","display_name":"Crotonyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fc5c708f-bac2-5eb3-84e5-0eda449fdcc9","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:gcdh-crotonyl-coa-event","event_type":"biochemical_relationship","label":"GCDH shortens the lysine-derived carbon chain.","description":"FAD-containing human GCDH dehydrogenates glutaryl-CoA through glutaconyl-CoA and decarboxylates it to crotonyl-CoA.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"27a3d8c4-8a68-5288-aa1e-6cbeb663f463","slug":"gcdh","display_name":"Glutaryl-CoA dehydrogenase / GCDH","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d4a5cca5-73c0-59b5-a1d3-4e45d7f3e69a","slug":"glutaryl-coa","display_name":"Glutaryl-CoA","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c4b21834-f44c-5037-9c3f-65c82f277137","slug":"glutaconyl-coa","display_name":"Glutaconyl-CoA","entity_type_key":"small_molecule"},"role":"intermediate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"c8ce07a8-dfe0-5fb9-9529-f4fe2fddc134","slug":"crotonyl-coa","display_name":"Crotonyl-CoA","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"966f14e5-c82f-5535-ad89-bfcc0adb2232","slug":"carbon-dioxide","display_name":"Carbon dioxide","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human GCDH crystallography and substrate-mechanism analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The downstream shared ECHS1/HADH/ACAT1 reactions are separate records; these individual enzyme assays do not measure the full lysine-derived flux in a person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"GCDH shortens the lysine-derived carbon chain.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fu2004] Crystal Structures of Human Glutaryl-CoA Dehydrogenase with and without an Alternate Substrate: Structural Bases of Dehydrogenation and Decarboxylation Reactions (2004). https://pubs.acs.org/doi/10.1021/bi049290c DOI: 10.1021/bi049290c","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial matrix","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b4d1583f-4108-5cab-a5e8-a78d9985a55a","evidence_kind":"source_excerpt","locator":"Lines 136-144","start_line":136,"end_line":144,"excerpt":"### gcdh-crotonyl-coa\nFAD-containing human GCDH dehydrogenates glutaryl-CoA through glutaconyl-CoA and decarboxylates it to crotonyl-CoA.\nPlain language: GCDH shortens the lysine-derived carbon chain.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Mitochondrial matrix\nexperimental_model: Human GCDH crystallography and substrate-mechanism analysis\nlimitations: The downstream shared ECHS1/HADH/ACAT1 reactions are separate records; these individual enzyme assays do not measure the full lysine-derived flux in a person.\n[fu2004] Crystal Structures of Human Glutaryl-CoA Dehydrogenase with and without an Alternate Substrate: Structural Bases of Dehydrogenation and Decarboxylation Reactions (2004). https://pubs.acs.org/doi/10.1021/bi049290c DOI: 10.1021/bi049290c","model_system":"Human GCDH crystallography and substrate-mechanism analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [fu2004] Crystal Structures of Human Glutaryl-CoA Dehydrogenase with and without an Alternate Substrate: Structural Bases of Dehydrogenation and Decarboxylation Reactions (2004). https://pubs.acs.org/doi/10.1021/bi049290c DOI: 10.1021/bi049290c","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}