{"id":"8ae60ccf-51ad-5333-b296-e742e0f42cad","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-c5-convertase-high","predicate":"decreases_in_recorded_comparison","statement":"C5-convertase inhibition required 1,500 micromolar RA in the tested system.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"1f666051-9495-51e9-b654-a0cec47d2a74","mechanism_event_label":"C5-convertase inhibition required 1,500 micromolar RA in the tested system.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"c0fbf82d-74cb-594c-b275-5e370a2232f4","slug":"human-c5-convertase","display_name":"Human complement C5 convertase, pathway specified by assay","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"1f666051-9495-51e9-b654-a0cec47d2a74","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-c5-convertase-high-event","event_type":"experimental_observation","label":"C5-convertase inhibition required 1,500 micromolar RA in the tested system.","description":"### ra-c5-convertase-high\n\nC5-convertase inhibition required 1,500 micromolar RA in the tested system.\nMuch higher concentration than C3b-attachment inhibition; not oral human target engagement.\nModel and exposure: Purified complement assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA at high concentration","sequence_order":0,"notes":""},{"entity":{"id":"c0fbf82d-74cb-594c-b275-5e370a2232f4","slug":"human-c5-convertase","display_name":"Human complement C5 convertase, pathway specified by assay","entity_type_key":"protein_complex"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA at high concentration\", \"comparator\": \"No RA\", \"endpoint\": \"C5-convertase inhibition required 1,500 micromolar RA in the tested system.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Purified complement assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"C5-convertase inhibition required 1,500 micromolar RA in the tested system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-c5-convertase-high; Much higher concentration than C3b-attachment inhibition; not oral human target engagement.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be21ac98-fc52-5be2-b59a-2fc0e5416257","evidence_kind":"source_excerpt","locator":"Lines 229-235","start_line":229,"end_line":235,"excerpt":"### ra-c5-convertase-high\n\nC5-convertase inhibition required 1,500 micromolar RA in the tested system.\nMuch higher concentration than C3b-attachment inhibition; not oral human target engagement.\nModel and exposure: Purified complement assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Purified complement assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}