{"id":"8aa39e17-69d4-5262-8f02-8877cfc09256","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-verapamil","predicate":"inhibits","statement":"Verapamil inhibited CAT1 arginine uptake with IC50 85.3 micromolar and an uncompetitive pattern.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"86e58d3f-6e52-5210-a33f-f4f979a17afa","mechanism_event_label":"A laboratory drug effect acts at the transport step.","subject":{"id":"6267a621-1246-5518-919d-132ea338dcfd","slug":"verapamil","display_name":"Verapamil","entity_type_key":"small_molecule"},"object":{"id":"19e0de57-73cc-5896-967b-0423c0f53277","slug":"cat1-arginine-uptake","display_name":"Human CAT1-mediated arginine uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"86e58d3f-6e52-5210-a33f-f4f979a17afa","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-verapamil-event","event_type":"observed_relationship","label":"A laboratory drug effect acts at the transport step.","description":"Verapamil inhibited CAT1 arginine uptake with IC50 85.3 micromolar and an uncompetitive pattern.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6267a621-1246-5518-919d-132ea338dcfd","slug":"verapamil","display_name":"Verapamil","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19e0de57-73cc-5896-967b-0423c0f53277","slug":"cat1-arginine-uptake","display_name":"Human CAT1-mediated arginine uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7fa43de-bc79-5059-b3ca-1a233156f4d1","slug":"slc7a1","display_name":"CAT1 / SLC7A1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full-text methods/results/discussion, Europe PMC PMC9217908.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Far above free therapeutic plasma exposure; the authors considered a significant clinical transport interaction unlikely.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A laboratory drug effect acts at the transport step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Screening of commonly prescribed drugs for effects on the CAT1-mediated transport of L-arginine and arginine derivatives. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35377022/ · DOI 10.1007/s00726-022-03156-2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e6944cc0-732f-564b-9f49-bb97a5998412","evidence_kind":"source_excerpt","locator":"Lines 46-52","start_line":46,"end_line":52,"excerpt":"## arg-verapamil\nA laboratory drug effect acts at the transport step.\nVerapamil inhibited CAT1 arginine uptake with IC50 85.3 micromolar and an uncompetitive pattern.\nModel: HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.\nLimitations: Far above free therapeutic plasma exposure; the authors considered a significant clinical transport interaction unlikely.\nEvidence access: Full-text methods/results/discussion, Europe PMC PMC9217908.\nScreening of commonly prescribed drugs for effects on the CAT1-mediated transport of L-arginine and arginine derivatives. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35377022/ · DOI 10.1007/s00726-022-03156-2","model_system":"HEK cells expressing human CAT1, compared with vector controls; radiolabeled substrate uptake.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}