{"id":"89fd5379-1512-5090-b21c-97a260e9debb","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-fibroblast-collagen-synthesis","predicate":"increases","statement":"Prolonged ascorbate exposure increased collagenous protein synthesis about eightfold in cultured human skin fibroblasts without a significant change in noncollagen protein synthesis.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0ce546f0-e603-5d7c-98d8-fd3437bda390","mechanism_event_label":"In this cell system vitamin C increased collagen production as well as supporting hydroxylation chemistry.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"8cfc1234-0fdd-5316-8bca-620c7d8f17d4","slug":"collagen-protein-synthesis","display_name":"Collagen protein synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0ce546f0-e603-5d7c-98d8-fd3437bda390","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-fibroblast-collagen-synthesis-event","event_type":"observed_intervention","label":"In this cell system vitamin C increased collagen production as well as supporting hydroxylation chemistry.","description":"Prolonged ascorbate exposure increased collagenous protein synthesis about eightfold in cultured human skin fibroblasts without a significant change in noncollagen protein synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"supporting-reductant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8cfc1234-0fdd-5316-8bca-620c7d8f17d4","slug":"collagen-protein-synthesis","display_name":"Collagen protein synthesis","entity_type_key":"cellular_process"},"role":"readout","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured human skin fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prolonged ascorbate exposure versus no added ascorbate; exact culture concentration not verified in accessible abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture exposure does not establish oral-dose efficacy, tissue-strength gain or an eightfold response in people; precise concentration/duration not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In this cell system vitamin C increased collagen production as well as supporting hydroxylation chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[collagen1981] Regulation of collagen synthesis by ascorbic acid. (1981). https://pubmed.ncbi.nlm.nih.gov/6265920/ DOI: 10.1073/pnas.78.5.2879","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skin fibroblasts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a09f1ce5-8e48-585e-bef1-96f0d3284e2e","evidence_kind":"source_excerpt","locator":"Lines 650-661","start_line":650,"end_line":661,"excerpt":"### vc-enzyme-fibroblast-collagen-synthesis\nProlonged ascorbate exposure increased collagenous protein synthesis about eightfold in cultured human skin fibroblasts without a significant change in noncollagen protein synthesis.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In this cell system vitamin C increased collagen production as well as supporting hydroxylation chemistry.\norganism: Homo sapiens\ntissue_or_cell_type: Skin fibroblasts\nexperimental_model: Cultured human skin fibroblasts\nlimitations: Culture exposure does not establish oral-dose efficacy, tissue-strength gain or an eightfold response in people; precise concentration/duration not extracted.\ncross_nutrient: Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.\nexposure: Prolonged ascorbate exposure versus no added ascorbate; exact culture concentration not verified in accessible abstract.\n[collagen1981] Regulation of collagen synthesis by ascorbic acid. 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