{"id":"89e2b1d9-9cc8-551d-b61e-a68824ca4915","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rat-sit1-ion-coupling","predicate":"imports","statement":"Rat SIT1 expressed in Xenopus oocytes transported proline with apparent K0.5 about 0.2 mM; transport depended on sodium, was stimulated by chloride and depended on voltage.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8bcb4d70-b72a-5314-8de2-c8651bfa3557","mechanism_event_label":"The driving ions and membrane voltage affect how this transporter works.","subject":{"id":"307de591-49a9-50cc-9321-61cebfe56bfa","slug":"rat-slc6a20","display_name":"Rat sodium/imino-acid transporter 1 / Slc6a20","entity_type_key":"protein"},"object":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8bcb4d70-b72a-5314-8de2-c8651bfa3557","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-rat-sit1-ion-coupling-event","event_type":"observed_relationship","label":"The driving ions and membrane voltage affect how this transporter works.","description":"Rat SIT1 expressed in Xenopus oocytes transported proline with apparent K0.5 about 0.2 mM; transport depended on sodium, was stimulated by chloride and depended on voltage.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"307de591-49a9-50cc-9321-61cebfe56bfa","slug":"rat-slc6a20","display_name":"Rat sodium/imino-acid transporter 1 / Slc6a20","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat protein in Xenopus oocytes; alanine and lysine did not inhibit the reported proline transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not a human dietary sodium or chloride threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The driving ions and membrane voltage affect how this transporter works.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15632147/ · DOI 10.1074/jbc.M413027200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Sodium-dependent, chloride-stimulated, voltage-dependent proline transport, which is inward.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Sodium-dependent, chloride-stimulated, voltage-dependent proline transport, which is inward.","entity":null}],"evidence":[{"id":"bb08275e-f2e1-5b34-9072-38de81327bce","evidence_kind":"source_excerpt","locator":"Lines 174-180","start_line":174,"end_line":180,"excerpt":"## l-proline-rat-sit1-ion-coupling\nThe driving ions and membrane voltage affect how this transporter works.\nRat SIT1 expressed in Xenopus oocytes transported proline with apparent K0.5 about 0.2 mM; transport depended on sodium, was stimulated by chloride and depended on voltage.\nModel: Rat protein in Xenopus oocytes; alanine and lysine did not inhibit the reported proline transport.\nLimitations: This is not a human dietary sodium or chloride threshold.\nEvidence access: Primary abstract\nIdentification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15632147/ · DOI 10.1074/jbc.M413027200","model_system":"Rat protein in Xenopus oocytes; alanine and lysine did not inhibit the reported proline transport.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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