{"id":"89dc6b41-b832-57cb-88e0-6b56e371eabc","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-arrest","predicate":"induces","statement":"Coumarin induced G0/G1 arrest in HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ee5f0511-52e5-5339-b29a-db7428786246","mechanism_event_label":"Exposed cells stopped progressing through part of their division cycle.","subject":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"object":{"id":"646eb5b9-cd7b-50c8-84d4-51f7d04fed6f","slug":"hela-g1-arrest","display_name":"G0/G1 arrest in human HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ee5f0511-52e5-5339-b29a-db7428786246","stable_key":"f812c44f-d0fd-5f01-ba2c-748c0794fcfb:coumarin-hela-arrest-event","event_type":"biochemical_relationship","label":"Exposed cells stopped progressing through part of their division cycle.","description":"Coumarin induced G0/G1 arrest in HeLa cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9fd9b93a-fdfd-5e09-92d3-0495bff41391","slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"646eb5b9-cd7b-50c8-84d4-51f7d04fed6f","slug":"hela-g1-arrest","display_name":"G0/G1 arrest in human HeLa cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coumarin-research/18210747.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\", \"start_char\": 0, \"end_char\": 1237, \"text_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell viability, cell cycle, protein and mitochondrial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Coumarin; reported viability IC50 54.2 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cultured cancer-cell response does not establish treatment efficacy; indexed abstract does not specify all exposure durations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coumarin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coumarin","display_name":"Coumarin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Exposed cells stopped progressing through part of their division cycle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coumarin-p18210747] Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation. (2007). https://pubmed.ncbi.nlm.nih.gov/18210747/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa cervical cancer cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b1ae0c0-eebd-58e7-9692-0c13fb369f6c","evidence_kind":"source_excerpt","locator":"Lines 319-330","start_line":319,"end_line":330,"excerpt":"### coumarin-hela-arrest\nCoumarin induced G0/G1 arrest in HeLa cells.\nCondition category: normal\nnutrient_topic: Coumarin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Exposed cells stopped progressing through part of their division cycle.\norganism: Human cell line\ntissue_or_cell_type: HeLa cervical cancer cells\nexperimental_model: Cell viability, cell cycle, protein and mitochondrial assays\nlimitations: Cultured cancer-cell response does not establish treatment efficacy; indexed abstract does not specify all exposure durations.\nexposure: Coumarin; reported viability IC50 54.2 micromolar\nevidence_span: {\"source_cache\": \"artifacts/coumarin-research/18210747.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\", \"start_char\": 0, \"end_char\": 1237, \"text_sha256\": \"e78df713d6bc2e34f13e80a0ef04c217a176b5d6082cbb28fa5a62464fcce842\"}\n[coumarin-p18210747] Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation. 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