{"id":"89c8a414-a523-583d-8b41-69ce9abb6f56","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-peptide-antibacterial","predicate":"enhances","statement":"Tartrazine increased DHVAR4 antibacterial activity in the tested cultures.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"556f46e6-e020-50f1-88ae-82ad8610e311","mechanism_event_label":"Tartrazine increased DHVAR4 antibacterial activity in the tested cultures.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"de819728-53d6-5b60-8f51-2cdbdf67d7fc","slug":"dhvar4-bacterial-killing-assay","display_name":"DHVAR4 antibacterial activity against tested Gram-positive and Gram-negative bacteria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"556f46e6-e020-50f1-88ae-82ad8610e311","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-peptide-antibacterial-event","event_type":"observed_relationship","label":"Tartrazine increased DHVAR4 antibacterial activity in the tested cultures.","description":"Tartrazine increased DHVAR4 antibacterial activity in the tested cultures.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"de819728-53d6-5b60-8f51-2cdbdf67d7fc","slug":"dhvar4-bacterial-killing-assay","display_name":"DHVAR4 antibacterial activity against tested Gram-positive and Gram-negative bacteria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b894725c-45b0-57aa-a5ae-f0de7cc5fbf0","slug":"dhvar4","display_name":"DHVAR4 antimicrobial peptide","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"DHVAR4 plus tartrazine; concentrations and ratios not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay intervals not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No clinical antimicrobial efficacy or effect on all natural histatins is established. Species-specific bactericidal thresholds and mammalian exposure require the full protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine increased DHVAR4 antibacterial activity in the tested cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anionic food color tartrazine enhances antibacterial efficacy of histatin-derived peptide DHVAR4 by fine-tuning its membrane activity. (2020). https://pubmed.ncbi.nlm.nih.gov/32115014/ DOI: 10.1017/S0033583520000013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro mixing and membrane/bacterial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Peptide-dye association and membrane activity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b670bd3a-69f6-5709-87ec-413ecdcf8bc5","evidence_kind":"source_excerpt","locator":"Lines 424-433","start_line":424,"end_line":433,"excerpt":"## tartrazine-peptide-antibacterial\nTartrazine increased DHVAR4 antibacterial activity in the tested cultures.\nModel/species: Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures\nTissue: Peptide-dye association and membrane activity\nExposure: DHVAR4 plus tartrazine; concentrations and ratios not specified in accessed abstract\nRoute: In vitro mixing and membrane/bacterial assays\nDuration: Assay intervals not specified in accessed abstract\nLimits: No clinical antimicrobial efficacy or effect on all natural histatins is established. Species-specific bactericidal thresholds and mammalian exposure require the full protocol.\nPrimary reference: Anionic food color tartrazine enhances antibacterial efficacy of histatin-derived peptide DHVAR4 by fine-tuning its membrane activity. (2020). https://pubmed.ncbi.nlm.nih.gov/32115014/ DOI: 10.1017/S0033583520000013\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}